02510naa a2200241 a 450000100080000000500110000800800410001902400250006010000120008524501590009726000090025650001140026552017070037965000110208665000220209765000110211970000170213070000160214770000150216370000170217870000170219577300560221210525702019-11-25       2014    bl uuuu     u00u1 u    #d7 a10.1071/AN123202DOI1 aGIL, J.  aThe leptin receptor is expressed in the Leydig cells of Merino rams, but its expression is not affected by long-term differences in the plane of nutrition  c2014  aArticle history: Received 12 September 2012 / Accepted 26 September 2013 / Published online 19 December 2013.  aABSTRACT.  We studied whether the long-term effects of nutrition on ram reproductive potential are mediated by changes in the expression and distribution of leptin receptor (Ob-Rb) in the testes. Thirty-two young Merino rams were allocated to a 2 × 2 factorial design, with either natural (NP) or improved pastures (IP), with (+S) or without (?S) supplementation. After 14 weeks of treatment, a blood sample was taken to measure leptin concentrations and rams were castrated. The weights of testicles (TW) and epididymides (EW) were recorded before tissue sampling for the detection of Ob-Rb expression by immunohistochemistry. TW was positively affected by the interaction IP × S, but not EW and bodyweight (BW), which were affected either by IP or S (P < 0.05). TW and EW were greatest (g) in IP+S rams (290.6 ± 40.1 and 57.6 ± 2.3), followed by IP?S (284.6 ± 61.4 and 52.5 ± 2.3), NP+S (282.6 ± 37.9 and 55.8 ± 2.3), and NP?S rams (208.5 ± 48.7 and 44.4 ± 2.3; P < 0.01). Expression of Ob-Rb was observed in the cytoplasm of Leydig cells, without any effect of treatments on the percentage of positive Leydig cells or on staining-intensity score. The serum leptin concentrations were similar among groups. The absolute volume of the seminiferous epithelium (mL) was affected by IP and S, as well as by their interaction (IP?S: 179.7 ± 13.1, IP+S; 174.9 ± 9.0; NP+S: 178.0 ± 10.2; NP?S: 120.8 ± 9.8; P < 0.05). We conclude that, under the conditions of the present study, the long-term improvement of nutrition promoted histological changes of increased spermatogenesis of Merino rams, but was not related to changes in the expression of the Ob-Rb receptor in the Leydig cell cytoplasm.  aMERINO  aNUTRICIÓN ANIMAL  aOVINOS1 aGENOVESE, P.1 aSARTORE, I.1 aBIELLI, A.1 aMONTOSSI, F.1 aVIÑOLES, C.  tAnimal Production Science, 2014gv. 54, p. 981-986.