02531naa a2200313 a 450000100080000000500110000800800410001902200140006002400350007410000160010924501290012526000090025452015920026365000230185565000220187865300210190065300260192165300190194765300280196665300220199465300220201665300110203870000180204970000220206770000160208970000180210570000190212377300750214210515242019-10-11       2009    bl uuuu     u00u1 u    #d  a0375-15897 a10.4314/sajas.v39i1.611572DOI1 aAGUERRE, M.  aRumen pH and NH3-N concentration of sheep fed temperate pastures and supplemented with sorghum grain.h[electronic resource]  c2009  aABSTRACT: The aim of this study was to evaluate the effect of sorghum grain supplementation on ruminal pH and NH3-N concentration of wethers consuming a fresh temperate pasture (Lotus corniculatus) in metabolism cages. Sixteen Corriedale x Milchschaf wethers were fed temperate pastures ad libitum and were non-supplemented or supplemented with ground sorghum grain at 5, 10 or 15 g/kg of their body weight (BW). Rumen fluid samples were collected at 0, 1, 2, 3, 4, 5 and 6 h after supplementation through permanent tubes inserted in the rumen. Ruminal pH was measured immediately and NH3-N concentration was determined by direct distillation. Mean daily pH values for non-supplemented wethers and supplemented with 5, 10 and 15 g/kg of their BW were 6.45, 6.14, 6.09 and 5.43, respectively. Significant differences in pH were found between the 15 g/kg supplemented group and non-supplemented, 5 and 10 g/kg supplemented groups, while a trend was found between non-supplemented and 10 g/kg supplemented group. After 0 h, all mean pH values for the non-supplemented group were above 6.15, while values for the 10 and 15 g/kg supplemented groups were below 6.2 and 6.0, respectively. No differences in NH3-N concentration among groups (mean = 37.15 mg/100 mL), between time or interaction between time and treatment were found. There was a correlation between pH and NH3-N when all measurements were considered. Ground sorghum grain supplementation significantly reduced rumen pH when 15 g/kg of BW was provided to wethers fed temperate pastures, but it did not affect NH3-N concentration.  aLOTUS CORNICULATUS  aNUTRICIÓN ANIMAL  aAMBIENTE RUMINAL  aGRAIN SUPPLEMENTATION  aGRANO DE SORGO  aNUTRICIÓN DE RUMIANTES  aRUMEN ENVIRONMENT  aTEMPERATE PASTURE  aWETHER1 aREPETTO, J.L.1 aPÉREZ-RUCHEL, A.1 aMENDOZA, A.1 aPINACCHIO, G.1 aCAJARVILLE, C.  tSouth African Journal of Animal Sciencegv.39, n.5., p. 246-250, 2009.