02728naa a2200289 a 450000100080000000500110000800800410001902200140006002400350007410000140010924501230012326000090024650001110025552017870036665000260215365000210217965000230220070000170222370000170224070000170225770000200227470000180229470000180231270000130233070000200234377300750236310512742021-06-24       2012    bl uuuu     u00u1 u    #d  a0925-98647 a10.1007/s10722-012-9806-x2DOI1 aREYNO, R.  aMolecular and cytogenetic characterization of a collection of bahiagrass (Paspalum notatum Flügge) native to Uruguay.  c2012  aArticle history: Received: 9 August 2011 / Accepted: 30 January 2012 / Published online: 22 February 2012.  aABSTRACT.  Paspalum notatum is a subtropical grass present throughout America, and one of the main constituents of the natural grasslands in Uruguay. An apomictic autotetraploid (2n = 4x = 40) is the most frequent cytotype. The occurrence of sexual diploids (2n = 2x = 20) has also been reported as well as the occasional presence of apomictic triploids and pentaploids in Argentina. In this study, ISSR (inter simple sequence repeats) molecular markers were used to analyze the genetic variability of 210 P. notatum individuals from a collection from Uruguay. Cytometric analyses and chromosome counts were used to assess the ploidy level of the individuals. All plants from Uruguay analyzed were tetraploid. Intra- and inter-population variability was found both in genomic DNA content and at the genotypic level. Several multilocus genotypes were shared among individuals within populations and among populations over moderate geographical ranges, at the same time, very dissimilar genotypes were found within the same population. Part of the genetic variance among populations can be explained by a broader scale geographic structure which is partly coincident with the traditionally recognized grassland management regions. In spite of the apparently high degree of genetic admixture within populations, groups of related genotypes seem to follow a broader geographical structure in the area under study. These results suggest that an efficient collection strategy for this apomictically reproducing species should include carefully planned intra- and inter-population sampling. A broader scale regional sampling strategy should also be considered although further studies will be required to define genetic structure at this level. © 2012 Springer Science+Business Media B.V.  aMARCADORES GENÉTICOS  aPASPALUM NOTATUM  aPLANTAS FORRAJERAS1 aNARANCIO, R.1 aSPERANZA, P.1 aDO CANTO, J.1 aLOPEZ-CARRO, B.1 aHERNANDEZ, P.1 aBURGUEÑO, J.1 aREAL, D.1 aDALLA RIZZA, M.  tGenetic Resources and Crop Evolution, 2012gv. 59, no.8, p. 1823-1832.