02158naa a2200313 a 450000100080000000500110000800800410001902200140006002400440007410000180011824501770013626000090031350000630032252011440038565000240152965000270155365000320158065000280161265000120164065300130165265300080166565300180167365300210169170000140171270000170172670000170174370000130176077300710177310505652019-10-14       2011    bl uuuu     u00u1 u    #d  a1593-20957 a10.14601/Phytopathol_Mediterr-91882DOI1 aNAVARRETE, F.  aPhatogenicity and molecular detection of Uruguayan isolates of Greneria uvicola and Cadophora luteo-olivacea associated with grapevine trunk disease.h[electronic resource]  c2011  aArticle history: Accepted for publication: April 25, 2011.  aSummary: Species from different fungal genera have been indicated as responsible for the development of trunk diseases of grapevines. Greeneria uvicola is responsible for the bitter rot of Vitis vinifera grape bunches near harvest, and can also attack other Vitis species. In Uruguay, G. uvicola was isolated from dead armaffected grapevines and as an endophyte from healthy canes. Cadophora luteo-olivacea is a phialophora-like ascomycete with a wide distribution that was isolated from asymptomatic wood tissues in Vitis and Petri disease-affected nursery plants in Uruguay. Pathogenicity of isolates of both species was evaluated on Vitis vinifera cv. Tannat and Cabernet Sauvignon, and rootstocks SO4 and 3309C. Specific primers were developed for the ITS rDNA region for both species. Number of plants showing discoloration, length of discoloration, number of re-isolations and amplifications confirmed the pathogenicity of G. uvicola isolates. Pathogenicity of the isolate of C. luteo-olivacea obtained from symptomatic tissues is discussed. Specific primers can be used to detect the presence of these fungi in asymptomatic tissues.  aENFERMEDAD DE PETRI  aENFERMEDADES DE LA VID  aENFERMEDADES DE LAS PLANTAS  aENFERMEDADES DEL TRONCO  aURUGUAY  aDEAD ARM  aITS  aPETRI DISEASE  aSPECIFIC PRIMERS1 aABREO, E.1 aMARTINEZ, S.1 aBETTUCCI, L.1 aLUPO, S.  tPhytopathologia Mediterranea. 2011.gv.50 (Supplement), p.166-175.