02874naa a2200313 a 450000100080000000500110000800800410001902200140006002400420007410000210011624502150013726000090035250000710036152018490043265000120228165000170229365000190231065000120232965300110234165300130235265300160236565300090238165300130239070000240240370000170242770000130244470000190245777300840247610327602019-10-10       2012    bl uuuu     u00u1 u    #d  a1439-03967 a10.1111/j.1439-0396.2011.01178.x2DOI1 aASTESSIANO, A.L.  aEffects of a short-term increase in the nutritional plane before the mating period on metabolic and endocrine parameters, hepatic gene expression and reproduction in primaparous beef cows on grazing conditions.  c2012  aArticle history: received: 23 February 2011; accepted: 5 May 2011.  aAbstract: Sixty-four spring-calved primiparous crossbred cows paired by calving date and body condition score (BCS) at calving were used to study the effect of a short-term increase in the nutritional plane before the mating period on cow and calf performance, changes in metabolic and endocrine parameters and hepatic gene expression. At 48 ± 10 days postpartum (onset of nutritional treatment = day 0), cows were assigned to two treatments during 23 days: control (grazing of native pastures; NP; n = 31) and increased nutritional plane (NP improved with Lotus subbiflorous cv Rincon; IP; n = 33). Cow body weight (BW), BCS and total protein and albumin concentrations increased while urea and non-esterified fatty acids levels decreased from the beginning of the nutritional treatment in both groups, indicating the animal positive energy balance as forage growth and availability of pastures increased during spring. In addition, cow BW and BCS, as well as calf average daily gain and BW, were greater in IP than in NP cows groups. Insulin concentrations were less in IP than in NP (1.37 vs. 2.25 ± 0.26 lU/ml) because insulin increased owing to nutritional treatment only in NP cows. Hepatic insulin receptor mRNA at day 23 tended to be 1.5-fold greater, while insulin growth factor binding protein-3 mRNA expression was 1.7-fold greater in NP than in IP cows. Reproductive responses were not affected by nutritional treatment, but days to initiation of ovarian ciclicity (108 ± 10 days) were positively correlated with insulin concentrations. Grazing of improved NP for 23 days before the mating period did not improve cow reproductive performance but modified metabolic, endocrine and gene expression parameters, in agreement with greater nutrient and energy partitioning towards milk production, reflected in better calf performance.  aBOVINOS  aREPRODUCCION  aSUPLEMENTACION  aURUGUAY  aCATTLE  aHORMONES  aMETABOLITES  aMRNA  aPASTURES1 aPÉREZ-CLARIGET, R.1 aQUINTANS, G.1 aSOCA, P.1 aCARRIQUIRY, M.  tJournal of Animal Physiology and Animal Nutrition, 2012gv.96, no.3, p.535-544.