01867naa a2200229 a 450000100080000000500110000800800410001902400430006010000140010324501320011726000090024950000720025852011120033065000110144265000290145365000280148270000150151070000160152570000120154170000130155377300710156610128082019-10-15       2011    bl uuuu     u00u1 u    #d7 a10.1016/j.postharvbio.2011.01.0022DOI1 aPEREZ, E.  aImazalil concentration for in vitro monitoring of imazalil resistant isolates of Penicillium digitatum in citrus packinghouses.  c2011  aArticle history: Received 30 August 2010 / Accepted 2 January 2011.  aABSTRACT.  The extensive use of imazalil (IMZ) in Uruguayan citrus packinghouses to control Penicillium spp. favored the selection and proliferation of resistant isolates. With the aim of detecting Penicillium digitatum biotypes that are not controlled by commercial doses of IMZ, the IMZ concentration within amended potato dextrose agar (PDA) plates was adjusted to 1.0mgL -1 IMZ. This concentration allowed the detection of resistant isolates that were not controlled by commercial applications of 3.0gL -1 IMZ. These isolates were able to grow in fruit with IMZ residues of 0.92 and 3.08mgkg -1. Therefore, environmental monitoring of facilities where commercial dip applications containing 3.0gL -1 IMZ are employed, should be done with 1.0mgL -1 of IMZ in Petri dishes. We can conclude that use of IMZ in postharvest applications of 3.0gL -1 remains effective to control wild Uruguayan P. digitatum isolates but not for resistant ones. A survey of 26 P. digitatum isolates collected in Uruguay indicated IMZ resistant isolates occurred in packinghouses and not in citrus groves.  © 2011 Elsevier B.V.  aCITRUS  aENFERMEDADES POSTCOSECHA  aPOSCOSECHA DE CÍTRICOS1 aBLANCO, O.1 aBERRETA, C.1 aDOL, I.1 aLADO, J.  tPostharvest Biology and Technology, 2011gv.60, no.3, p. 258-262,.