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| Registros recuperados : 6 | |
| 2. |  | SESSA, L.; PEDRINI, N.; ALTIER, N.; ABREO, E. Conociendo al enemigo: Hidrocarburos epicuticulares de insectos plaga y su utilización para el biocontrol. O11. Módulo 3: Nuevas estrategias para potenciar el control biológico. In: Abreo, E.; Beyhaut, E.; Rivas, F. (Eds.). Simposio Microorganismos para la Agricultura, 2. [Resúmenes y Posters]. Canelones (UY): INIA, 2022. p.22. (Serie Actividades de Difusión; 801). Financiamiento: Beca ANII, INIA, Beca CAP.| Biblioteca(s): INIA Las Brujas. |
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| 4. | | SESSA, L.; PEDRINI, N.; ALTIER, N.; ABREO, E. Hidrocarburos epicuticulares de Piezodorus guildinii: efecto de los insecticidas y uso de n-pentadecano para aumentar la virulencia de cepas de Beauveria bassiana. In: Sociedad Uruguaya de Fitopatología Jornada Uruguaya de Fitopatología, 6., Jornada Uruguaya de Protección Vegetal, 4., 21-22 octubre, 2021, Montevideo, Uruguay. Libro de resúmenes. Montevideo (UY): Sociedad Uruguay de Fitopatología (SUFIT), 2021. p. 70 Fianciamiento: INIA-ANII-PICT.| Biblioteca(s): INIA Treinta y Tres. |
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| 5. | | ABREO, E.; GIROTTI, J.; MIJAILOVSKY, S.; JUÁREZ, P.; ALTIER, N.; ZERBINO, M.; PEDRINI, N. Determinación de los hidrocarburos de la epicutícula de insectos plaga como herramienta para la selección y producción masiva de agentes microbianos de control biológico. In: JORNADA NACIONAL DE FITOPATOLOGÍA, 3; JORNADA NACIONAL DE PROTECCIÓN VEGETAL, 1., 3 SETIEMBRE 2015, MONTEVIDEO, URUGUAY. Libro de Resúmenes. Montevideo (Uruguay) : SUFIT, 2015. p. 43| Biblioteca(s): INIA Las Brujas. |
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| 6. | | SESSA, L.; CALDERÓN-FERNÁNDEZ, G.M.; ABREO, E.; ALTIER, N.; MIJAILOVSKY, S.J.; GIROTTI, J.R.; PEDRINI, N. Epicuticular hydrocarbons of the redbanded stink bug Piezodorus guildinii (Heteroptera: Pentatomidae): sexual dimorphism and alterations in insects collected in insecticide-treated soybean crops. Pest Management Science, 2021. Doi: https://doi.org/10.1002/ps.6528 Article history: Received 8 June 2021, Accepted article published, 24 June 2021.
This work was financed by Agencia Nacional de Investigación e Innovación (ANII) grant POS_NAC_2016_1_130245 and Instituto Nacional de Investigación...| Biblioteca(s): INIA Las Brujas. |
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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
20/02/2024 |
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Actualizado : |
20/02/2024 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
SESSA, L.; OBERTI, H.; ABREO, E.; PEDRINI, N. |
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Afiliación : |
LUCÍA OLGA SESSA JUSID, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.; HÉCTOR OBERTI RVAROLA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; EDUARDO RAUL ABREO GIMENEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; NICOLÁS PEDRINI, Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), CCT La Plata Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)-Universidad Nacional de La Plata (UNLP), calles 60 y 120, La Plata, 1900, Argentina. |
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Título : |
Beauveria bassiana transcriptomics reveal virulence-associated shifts during insect lipid assimilation. |
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Fecha de publicación : |
2024 |
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Fuente / Imprenta : |
Applied Microbiology and Biotechnology. 2024; 108(1): 1-17. https://doi.org/10.1007/s00253-023-12898-2 |
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ISSN : |
0175-7598 |
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DOI : |
10.1007/s00253-023-12898-2 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 04 July 2023; Revised 19 September 2023; Accepted 03 October 2023; Published 30 December 2023. -- Lucia Sessa and Héctor Oberti share first authorship. -- Correspondence authors: Abreo, E.; Laboratorio de Bioproducción, Plataforma de Bioinsumos. Instituto Nacional de Investigación Agropecuaria, Estación Experimental Wilson Ferreira Aldunate, Ruta 48, km, Canelones, Uruguay; email:eabreo@inia.org.uy ; Pedrini, N.; Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), CCT La Plata Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)-Universidad Nacional de La Plata (UNLP), calles 60 y 120, La Plata, Argentina; email:npedrini@med.unlp.edu.ar -- Supplementary information: https://static-content.springer.com/esm/art%3A10.1007%2Fs00253-023-12898-2/MediaObjects/253_2023_12898_MOESM1_ESM.pdf -- Funding: This work was financed by Comisión Académica de Posgrado (CAP) and Instituto Nacional de Investigación Agropecuaria (INIA, Uruguay) Projects SA24 and SA47. -- Reaxys Chemistry database information. Substances: Pentadecane. -- Publisher: Springer Science and Business Media Deutschland GmbH. -- |
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Contenido : |
ABSTRACT.- Insect cuticular lipids, especially epicuticular hydrocarbons (CHC), have a significant role in insect ecology and interactions with other organisms, including fungi. The CHC composition of a specific insect species may influence the outcome of the interaction with a specific fungal strain. Some insects, such as Piezodorus guildinii, have low susceptibility towards fungal infections seemingly due to their CHC composition. The entomopathogenic fungus Beauveria bassiana can assimilate CHC and incorporate them as building blocks via cytochrome P450 monooxygenases (CYPs). However, little is known about other enzymes that promote the degradation/assimilation of these cuticular components. In this study, we performed a transcriptomic analysis to evaluate the in vitro response of two virulence-contrasting B. bassiana strains when grown on three different P. guildinii CHC sources. We found a different expression profile of virulence-related genes, as well as different GO and KEGG parameters enriched at 4 days post-inoculation, which could help account for the intrinsic virulence and for an alkane-priming virulence enhancement effect. The hypovirulent strain predominantly showed higher expression of cuticle penetration genes, including chitinases, proteases, and CYPs, with GO term categories of "heme binding," "monooxygenase activity," and "peroxisome" pathways enriched. The hypervirulent strain showed higher expression of cell wall remodeling and cell cycle genes, and cuticle adhesion and a distinct set of CYPs, with GO categories of "DNA-binding transcription factor activity" and KEGG pathways corresponding to "meiosis-yeast" and "cell cycle" enriched. These results suggest a delay and alternate routes in pathogenicity-related metabolism in the hypovirulent strain in comparison with the hypervirulent strain. Key points: ?Transcriptomics of two B. bassiana strains grown in P. guildinii cuticular components ?Virulence-related genes correlated with virulence enhancement towards P. guildinii ?Differentially expressed genes, GOs and KEGGs showed different metabolic timelines associated with virulence. © 2023, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. MenosABSTRACT.- Insect cuticular lipids, especially epicuticular hydrocarbons (CHC), have a significant role in insect ecology and interactions with other organisms, including fungi. The CHC composition of a specific insect species may influence the outcome of the interaction with a specific fungal strain. Some insects, such as Piezodorus guildinii, have low susceptibility towards fungal infections seemingly due to their CHC composition. The entomopathogenic fungus Beauveria bassiana can assimilate CHC and incorporate them as building blocks via cytochrome P450 monooxygenases (CYPs). However, little is known about other enzymes that promote the degradation/assimilation of these cuticular components. In this study, we performed a transcriptomic analysis to evaluate the in vitro response of two virulence-contrasting B. bassiana strains when grown on three different P. guildinii CHC sources. We found a different expression profile of virulence-related genes, as well as different GO and KEGG parameters enriched at 4 days post-inoculation, which could help account for the intrinsic virulence and for an alkane-priming virulence enhancement effect. The hypovirulent strain predominantly showed higher expression of cuticle penetration genes, including chitinases, proteases, and CYPs, with GO term categories of "heme binding," "monooxygenase activity," and "peroxisome" pathways enriched. The hypervirulent strain showed higher expression of cell wall remodeling and cell cycle genes, and cut... Presentar Todo |
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Palabras claves : |
Alkanes; Cuticle; Gene expression; Hydrocarbons; Piezodorus guildinii; PLATAFORMA DE BIOINSUMOS - INIA. |
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Asunto categoría : |
-- |
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Marc : |
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022 $a0175-7598
024 7 $a10.1007/s00253-023-12898-2$2DOI
100 1 $aSESSA, L.
245 $aBeauveria bassiana transcriptomics reveal virulence-associated shifts during insect lipid assimilation.$h[electronic resource]
260 $c2024
500 $aArticle history: Received 04 July 2023; Revised 19 September 2023; Accepted 03 October 2023; Published 30 December 2023. -- Lucia Sessa and Héctor Oberti share first authorship. -- Correspondence authors: Abreo, E.; Laboratorio de Bioproducción, Plataforma de Bioinsumos. Instituto Nacional de Investigación Agropecuaria, Estación Experimental Wilson Ferreira Aldunate, Ruta 48, km, Canelones, Uruguay; email:eabreo@inia.org.uy ; Pedrini, N.; Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), CCT La Plata Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)-Universidad Nacional de La Plata (UNLP), calles 60 y 120, La Plata, Argentina; email:npedrini@med.unlp.edu.ar -- Supplementary information: https://static-content.springer.com/esm/art%3A10.1007%2Fs00253-023-12898-2/MediaObjects/253_2023_12898_MOESM1_ESM.pdf -- Funding: This work was financed by Comisión Académica de Posgrado (CAP) and Instituto Nacional de Investigación Agropecuaria (INIA, Uruguay) Projects SA24 and SA47. -- Reaxys Chemistry database information. Substances: Pentadecane. -- Publisher: Springer Science and Business Media Deutschland GmbH. --
520 $aABSTRACT.- Insect cuticular lipids, especially epicuticular hydrocarbons (CHC), have a significant role in insect ecology and interactions with other organisms, including fungi. The CHC composition of a specific insect species may influence the outcome of the interaction with a specific fungal strain. Some insects, such as Piezodorus guildinii, have low susceptibility towards fungal infections seemingly due to their CHC composition. The entomopathogenic fungus Beauveria bassiana can assimilate CHC and incorporate them as building blocks via cytochrome P450 monooxygenases (CYPs). However, little is known about other enzymes that promote the degradation/assimilation of these cuticular components. In this study, we performed a transcriptomic analysis to evaluate the in vitro response of two virulence-contrasting B. bassiana strains when grown on three different P. guildinii CHC sources. We found a different expression profile of virulence-related genes, as well as different GO and KEGG parameters enriched at 4 days post-inoculation, which could help account for the intrinsic virulence and for an alkane-priming virulence enhancement effect. The hypovirulent strain predominantly showed higher expression of cuticle penetration genes, including chitinases, proteases, and CYPs, with GO term categories of "heme binding," "monooxygenase activity," and "peroxisome" pathways enriched. The hypervirulent strain showed higher expression of cell wall remodeling and cell cycle genes, and cuticle adhesion and a distinct set of CYPs, with GO categories of "DNA-binding transcription factor activity" and KEGG pathways corresponding to "meiosis-yeast" and "cell cycle" enriched. These results suggest a delay and alternate routes in pathogenicity-related metabolism in the hypovirulent strain in comparison with the hypervirulent strain. Key points: ?Transcriptomics of two B. bassiana strains grown in P. guildinii cuticular components ?Virulence-related genes correlated with virulence enhancement towards P. guildinii ?Differentially expressed genes, GOs and KEGGs showed different metabolic timelines associated with virulence. © 2023, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
653 $aAlkanes
653 $aCuticle
653 $aGene expression
653 $aHydrocarbons
653 $aPiezodorus guildinii
653 $aPLATAFORMA DE BIOINSUMOS - INIA
700 1 $aOBERTI, H.
700 1 $aABREO, E.
700 1 $aPEDRINI, N.
773 $tApplied Microbiology and Biotechnology. 2024; 108(1): 1-17. https://doi.org/10.1007/s00253-023-12898-2
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