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| Registros recuperados : 7 | |
| 1. |  | SARAVIA, A.; MIRABALLES, C.; RIET-CORREA, F.; CASTRO-JANER, E. Eimeria spp. in dairy calves in Uruguay. Identification, dynamics of oocyst excretion and association with the age of calves. Veterinary Parasitology: Regional Studies and Reports, 25 July 2021, Volume 25, Article number 100588. Doi: https://doi.org/10.1016/j.vprsr.2021.100588. Article history: Received 12 February 2020, Revised 19 February 2021, Accepted 12 May 2021, Available online 14 May 2021.| Biblioteca(s): INIA La Estanzuela. |
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| 2. | | MIRABALLES, C.; BUSCIO, D.; SARAVIA, A.; DIAZ, A.; CASTRO-JANER, E. Comportamiento de vacas en ordeñe ante una alternativa de control no químico de la "Mosca de los cuernos" , Haematobia Irritans. In: Jornadas Uruguayas de Buiatría, 44., 2016, Paysandú, UY.; Gianneechini, E.; Elizondo, V. (Ed.).Paysandú: Centro Médico Veterinario de Paysandú/Sociedad Uruguaya de Buiatría, 2016. p.219-221.| Biblioteca(s): INIA La Estanzuela. |
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| 5. | | CASTRO JANER, E.; DÍAZ, A.; FONTES, F.; BARAIBAR, F.; SAPORITI, T.; OLHAGARAY, M. E. Molecular survey of pyrethroid and fipronil resistance in isolates of Rhipicephalus microplus in the north of Uruguay. Ticks and Tick-borne Diseases, 2021, Volume 12, Issue 5, Article number 101747. Doi: https://doi.org/10.1016/j.ttbdis.2021.101747 Article history: Received 23 July 2020; Received in revised form 29 March 2021; Accepted 6 April 2021; Available online 19 May 2021.
The authors are grateful to the funding of the Instituto Nacional de Investigación...| Biblioteca(s): INIA Las Brujas. |
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| 7. | | CASTRO JANER, E.; SATO SCHUMAKER, T.T.; MARCONDES KLAFKE, G.; RIFRAN, L.; GONZÁLEZ, P.; NIELL, C.; NAMINDOME, A.; GIL, A.; PIAGGIO, J.; MARTINS, J.R.; MENDES, M.C.; MILLER, R.J. GARRAPATA: resistencia a Fipronil e Ivermectina en rodeos vacunos de Uruguay y Brasil Montevideo (UY): INIA, 2012. 69 p. (Serie FPTA-INIA; 35) "Proyecto FPTA 243 ""Adecuación de bioensayos para la determinación de resistencia de Boophilus microplus a fipronil e ivermectina, y verificación de resistencia cruzada entre ambas drogas"". Período de Ejecución: Dic. 2006-Nov. 2009"| Biblioteca(s): INIA Las Brujas; INIA Tacuarembó. |
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| Registros recuperados : 7 | |
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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
30/06/2021 |
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Actualizado : |
30/06/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
CASTRO JANER, E.; DÍAZ, A.; FONTES, F.; BARAIBAR, F.; SAPORITI, T.; OLHAGARAY, M. E. |
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Afiliación : |
ELEONOR CASTRO JANER, Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Montevideo, Uruguay.; ANDRÉ DÍAZ, Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Montevideo, Uruguay.; FLORENCIA FONTES, Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Montevideo, Uruguay.; FLORENCIA BARAIBAR, Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Montevideo, Uruguay.; TATIANA SAPORITI NOGUEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA ERNESTINA OLHAGARAY, Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Montevideo, Uruguay. |
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Título : |
Molecular survey of pyrethroid and fipronil resistance in isolates of Rhipicephalus microplus in the north of Uruguay. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Ticks and Tick-borne Diseases, 2021, Volume 12, Issue 5, Article number 101747. Doi: https://doi.org/10.1016/j.ttbdis.2021.101747 |
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ISSN : |
1877-959X |
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DOI : |
10.1016/j.ttbdis.2021.101747 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 23 July 2020; Received in revised form 29 March 2021; Accepted 6 April 2021; Available online 19 May 2021.
The authors are grateful to the funding of the Instituto Nacional de Investigación Agropecuaria-Plataforma de Salud Animal-Uruguay (CL_35).
Corresponding author: Janer, E.C.; Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Av. Lasplaces 1620, Montevideo, CP, Uruguay; email:elinorcastro59@gmail.com |
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Contenido : |
ABSTRACT - The resistance of Rhipicephalus microplus to acaricides is a serious control problem, so its early diagnosis by a molecular technique is important. This study aims to develop a multiplex allele?specific polymerase chain reaction (PCR) for single-nucleotide polymorphisms (SNPs) in the para-sodium channel gene and in the GABA-Cl gene, associated with pyrethroids (cypermethrin and flumethrin) and fipronil resistance, respectively. We used 22 tick field isolates from farms with tick control problems (sampling convenience). These farms are located in departments of northern Uruguay. Three mutations in the sodium channel gene (Domain II S4?5: C190A and G215T; domain III S6: T2134A) and one in the GABA-Cl gene (A286S/L: CG856CC/TG) were studied. Mutations G215T and T213A were not detected. In all field isolates, the resistant allele (R) for C190A mutation (knockdown resistance, kdr) was detected, mainly in heterozygous individuals (SR) (11.1% to 86.7%). The highest incidence of the kdr mutant allele occurred in the Tacuarembó tick field isolates, where on 7 out of 10 farms >30% of individuals were SR and on one farm > 30% of individuals were RR. The next highest was Artigas (half of farms had>30% SR individuals and a quarter had >30% RR individuals). The resistance to dieldrin locus (rdl) mutation (CG856CC/TG) was absent in five field isolates. The highest incidenceof the mutant allele was observed in ticks from farms in Rivera (all farms had SR in >30% of individuals and two farms had RR in >12.5 and >16.7% of individuals) followed by farms in Tacuarembó (3 of 10 farms had >30% SR and 2 with >30% RR). Less than half of the farms had rdl in homozygous individuals. No significant association was observed between phenotypic bioassays and the rdl resistance allele. Several field isolates were phenotypically susceptible to the presence of the rdl allele. Several causes are possible (bioassay sensitivity, discriminating concentration). Individuals with simultaneous kdr and rdl mutations were present in 17 field isolates, and their frequency varied between 0.06% and 60%. Genotypic analysis shows that tick resistance to both acaricides, especially pyrethroids, is a serious problem. It is important to monitor the resistance using molecular techniques to plan efficient control measures. This is the first report describing kdr and rdl detection in R. microplus in Uruguay. © 2021 MenosABSTRACT - The resistance of Rhipicephalus microplus to acaricides is a serious control problem, so its early diagnosis by a molecular technique is important. This study aims to develop a multiplex allele?specific polymerase chain reaction (PCR) for single-nucleotide polymorphisms (SNPs) in the para-sodium channel gene and in the GABA-Cl gene, associated with pyrethroids (cypermethrin and flumethrin) and fipronil resistance, respectively. We used 22 tick field isolates from farms with tick control problems (sampling convenience). These farms are located in departments of northern Uruguay. Three mutations in the sodium channel gene (Domain II S4?5: C190A and G215T; domain III S6: T2134A) and one in the GABA-Cl gene (A286S/L: CG856CC/TG) were studied. Mutations G215T and T213A were not detected. In all field isolates, the resistant allele (R) for C190A mutation (knockdown resistance, kdr) was detected, mainly in heterozygous individuals (SR) (11.1% to 86.7%). The highest incidence of the kdr mutant allele occurred in the Tacuarembó tick field isolates, where on 7 out of 10 farms >30% of individuals were SR and on one farm > 30% of individuals were RR. The next highest was Artigas (half of farms had>30% SR individuals and a quarter had >30% RR individuals). The resistance to dieldrin locus (rdl) mutation (CG856CC/TG) was absent in five field isolates. The highest incidenceof the mutant allele was observed in ticks from farms in Rivera (all farms had SR in >30% of individuals an... Presentar Todo |
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Palabras claves : |
Acaricides; Ixodidae; Reaxys retrieves substances; Rhipicephalus microplus. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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Marc : |
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001 1062207
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022 $a1877-959X
024 7 $a10.1016/j.ttbdis.2021.101747$2DOI
100 1 $aCASTRO JANER, E.
245 $aMolecular survey of pyrethroid and fipronil resistance in isolates of Rhipicephalus microplus in the north of Uruguay.$h[electronic resource]
260 $c2021
500 $aArticle history: Received 23 July 2020; Received in revised form 29 March 2021; Accepted 6 April 2021; Available online 19 May 2021. The authors are grateful to the funding of the Instituto Nacional de Investigación Agropecuaria-Plataforma de Salud Animal-Uruguay (CL_35). Corresponding author: Janer, E.C.; Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Av. Lasplaces 1620, Montevideo, CP, Uruguay; email:elinorcastro59@gmail.com
520 $aABSTRACT - The resistance of Rhipicephalus microplus to acaricides is a serious control problem, so its early diagnosis by a molecular technique is important. This study aims to develop a multiplex allele?specific polymerase chain reaction (PCR) for single-nucleotide polymorphisms (SNPs) in the para-sodium channel gene and in the GABA-Cl gene, associated with pyrethroids (cypermethrin and flumethrin) and fipronil resistance, respectively. We used 22 tick field isolates from farms with tick control problems (sampling convenience). These farms are located in departments of northern Uruguay. Three mutations in the sodium channel gene (Domain II S4?5: C190A and G215T; domain III S6: T2134A) and one in the GABA-Cl gene (A286S/L: CG856CC/TG) were studied. Mutations G215T and T213A were not detected. In all field isolates, the resistant allele (R) for C190A mutation (knockdown resistance, kdr) was detected, mainly in heterozygous individuals (SR) (11.1% to 86.7%). The highest incidence of the kdr mutant allele occurred in the Tacuarembó tick field isolates, where on 7 out of 10 farms >30% of individuals were SR and on one farm > 30% of individuals were RR. The next highest was Artigas (half of farms had>30% SR individuals and a quarter had >30% RR individuals). The resistance to dieldrin locus (rdl) mutation (CG856CC/TG) was absent in five field isolates. The highest incidenceof the mutant allele was observed in ticks from farms in Rivera (all farms had SR in >30% of individuals and two farms had RR in >12.5 and >16.7% of individuals) followed by farms in Tacuarembó (3 of 10 farms had >30% SR and 2 with >30% RR). Less than half of the farms had rdl in homozygous individuals. No significant association was observed between phenotypic bioassays and the rdl resistance allele. Several field isolates were phenotypically susceptible to the presence of the rdl allele. Several causes are possible (bioassay sensitivity, discriminating concentration). Individuals with simultaneous kdr and rdl mutations were present in 17 field isolates, and their frequency varied between 0.06% and 60%. Genotypic analysis shows that tick resistance to both acaricides, especially pyrethroids, is a serious problem. It is important to monitor the resistance using molecular techniques to plan efficient control measures. This is the first report describing kdr and rdl detection in R. microplus in Uruguay. © 2021
653 $aAcaricides
653 $aIxodidae
653 $aReaxys retrieves substances
653 $aRhipicephalus microplus
700 1 $aDÍAZ, A.
700 1 $aFONTES, F.
700 1 $aBARAIBAR, F.
700 1 $aSAPORITI, T.
700 1 $aOLHAGARAY, M. E.
773 $tTicks and Tick-borne Diseases, 2021, Volume 12, Issue 5, Article number 101747. Doi: https://doi.org/10.1016/j.ttbdis.2021.101747
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