Fecha/hora: 16/06/2024 06:11:39

Biblioteca(s): 	INIA Las Brujas.	
Fecha corriente: 	11/08/2016	
Fecha de la última actualización: 	15/10/2019	
Autoria: 	TAULÉ, C.; CASTILLO, A.; VILLAR, S.; OLIVARES, F.; BATTISTONI, F.	
Afiliación: 	CECILIA TAULÉ, MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); ALICIA MARIA CASTILLO SALLE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SILVIA VILLAR, Universidad de la República (UdelaR)/ Facultad de Ciencias; FABIO OLIVARES, Laboratory of Cell and Tissue Biology, North Fluminense State University Darcy Ribeiro; FEDERICO BATTISTONI, MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable").	
Título: 	Endophytic colonization of sugarcane (Saccharum officinarum) by the novel diazotrophs Shinella sp. UYSO24 and Enterobacter sp. UYSO10.	
Año de publicación: 	2016	
Fuente/Imprenta: 	Plant and Soil, 2016, v. 403, no. 1, p. 403?418.	
DOI: 	10.1007/s11104-016-2813-5	
Idioma: 	Inglés	
Notas: 	Article history: Received: 9 March 2015 /Accepted: 22 January 2016 / Published online: 3 February 2016	
Contenido: 	ABSTRACT.

Aims Sugarcane is a multipurpose crop mostly used in Uruguay for bioethanol production. It requires high amounts of N fertilization for optimal growth, which causes environmental degradation and high production costs. Previously, a bacterial collection associated with surface-sterilized stems of sugarcane was characterized for in vitro plant growth-promoting (PGP) traits. The aims of this study were (1) to determine if selected isolates from the collection are sugarcane growth promoters and (2) to determine if they are true endophytes
of sugarcane. Methods Plant growth promotion assays were used to study the effects of selected isolates on sugarcane plantlets. Light microscopy, transmission electron, and scanning electron microscopy (TEM, SEM) were employed to describe the structure of the interaction between the plant growth-promoting bacteria and the plants. qPCR was used to quantify the bacteria residing in the inner plant tissues. Results Enterobacter sp. UYSO10 and Shinella sp. UYSO24 were confirmed to have a PGP effect on the commercial sugarcane cv. LCP 85384. Both strains were defined as true endophytes of sugarcane plants with this being the first case for a strain in the genus Shinella in grasses. Conclusions These data will contribute to the final development of a sugarcane PGP inoculant based on endophytic plant growth-promoting bacteria.

@Springer International Publishing Switzerland 2016	
Palabras-Clave: 	ELECTRON MICROSCOPY; ENDOPHYTE; ENTEROBACTER. SHINELLA; PLANT GROWTH PROMOTION; SUGARCANE.	
Thesagro: 	CANA DE AZUCAR.	
Categoría del tema: 	--	
Registro original: 	INIA Las Brujas (LB)	
Ejemplares: 	LB - (101071-1) - PXI - AP - DD -  -  -  - DIS