Fecha/hora: 07/06/2024 13:07:15

Biblioteca(s): | INIA Las Brujas.
Tipo de la producción científica: | Capítulo en Libro Técnico-Científico
Fecha corriente: | 01/11/2021
Fecha de la última actualización: | 01/11/2021
Autoria: | FERREIRA, V.; GONZÁLEZ-ARCOS, M.; PIANZZOLA, M.J.; COLL, N.S.; SIRI, M.I.; VALLS, M.
Afiliación: | VIRGINIA FERREIRA, Área Microbiología, Departamento de Biociencias (DEPBIO), Facultad de Química, Universidad de la República, Montevideo, Uruguay; MATIAS GONZÁLEZ-ARCOS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA JULIA PIANZZOLA, Área Microbiología, Departamento de Biociencias (DEPBIO), Facultad de Química, Universidad de la República, Montevideo, Uruguay; NÚRIA S. COLL, Centre for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Bellaterra, Catalonia, Spain; MARÍA INÉS SIRI, Área Microbiología, Departamento de Biociencias (DEPBIO), Facultad de Química, Universidad de la República, Montevideo, Uruguay; MARC VALLS, Centre for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Bellaterra, Catalonia, Spain;  Department of Genetics, University of Barcelona, Bellaterra, Catalonia, Spain.
Título: | Molecular detection of Ralstonia solanacearum to facilitate breeding for resistance to bacterial wilt in potato.
Año de publicación: | 2021
Fuente/Imprenta: | In: Dobnik D., Gruden K., Ram?ak ?., Coll A. (eds). Solanum tuberosum. Methods in Molecular Biology, 2021, vol 2354. Humana, New York, NY. doi: https://doi.org/10.1007/978-1-0716-1609-3_18
Série: | eBook Packages Springer Protocols, (Methods in Molecular Biology, volume 2354).
ISBN: | 978-1-0716-1608-6 (print) / 978-1-0716-1609-3 (e-book)
ISSN: | 1064-3745 (print) / 1940-6029 (electronic)
DOI: | 10.1007/978-1-0716-1609-3_18
Idioma: | Inglés
Notas: | Article history: First Online 27 August 2021.
Contenido: | ABSTRACT. - Potato bacterial wilt is caused by the devastating bacterial pathogen Ralstonia solanacearum. Quantitative resistance to this disease has been and is currently introgressed from a number of wild relatives into cultivated varieties through laborious breeding programs. Here, we present two methods that we have developed to facilitate the screening for resistance to bacterial wilt in potato. The first one uses R. solanacearum reporter strains constitutively expressing the luxCDABE operon or the green fluorescent protein (gfp) to follow pathogen colonization in potato germplasm. Luminescent strains are used for nondestructive live imaging, while fluorescent ones enable precise pathogen visualization inside the plant tissues through confocal microscopy. The second method is a BIO-multiplex-PCR assay that is useful for sensitive and specific detection of viable R. solanacearum (IIB-1) cells in latently infected potato plants. This BIO-multiplex-PCR assay can specifically detect IIB-1 sequevar strains as well as strains belonging to all four R. solanacearum phylotypes and is sensitive enough to detect without DNA extraction ten bacterial cells per mL in complex samples. The described methods allow the detection of latent infections in roots and stems of asymptomatic plants and were shown to be efficient tools to assist potato breeding programs. © 2021, Springer Science+Business Media, LLC, part of Springer Nature.
Palabras-Clave: | Bacterial wilt; Disease resistance; Plant breeding; Potato brown rot; Ralstonia solanacearum; Solanum tuberosum.
Categoría del tema: | F30 Genética vegetal y fitomejoramiento
Registro original: | INIA Las Brujas (LB)
Ejemplares: | LB - (102827-1) - PXI - PL - DD - Methods in Molecular Biology/2354/2021 -  -  - DIS