Fecha/hora: 31/05/2024 21:04:11

Biblioteca(s): | INIA Las Brujas.
Tipo de la producción científica: | Artículos Indexados
Fecha corriente: | 25/03/2021
Fecha de la última actualización: | 25/03/2021
Autoria: | NASCIMENTO, D. C.; DINI, M.; SAMPAIO, N. V.; SCHUCH, M. W.
Afiliación: | DANIELE CAMARGO NASCIMENTO, Universidade Federal do Pampa/UNIPAMPA, Dom Pedrito, RS, Brasil; MAXIMILIANO ANTONIO DINI VIÑOLY, Universidade Federal de Pelotas/UFPel, Capão do Leão, RS, Brasil; NORTON VICTOR SAMPAIO, Universidade Federal do Pampa/UNIPAMPA, Dom Pedrito, RS, Brasil; MÁRCIA WULFF SCHUCH, Universidade Federal de Pelotas/UFPel, Capão do Leão, RS, Brasil.
Título: | In vitro multiplication and rooting of grapevine; culture media and plant growth regulators.
Año de publicación: | 2019
Fuente/Imprenta: | Plant Cell Culture & Micropropagation, 2019, volume 15, n. 1, p. 1-7. OPEN ACCESS. Doi: https://doi.org/10.46526/pccm.2019.v15i1.136
ISSN: | 1808-9909
DOI: | 10.46526/pccm.2019.v15i1.136
Idioma: | Inglés
Notas: | Article history: Received in July, 2019 and approved in April, 2020.

Contenido:

ABSTRACT.
Micropropagation is a technique that allows to obtain quality plants, making it an important tool in the production of grapevine matrices. Composition of the culture media and plant growth regulators are commonly tested to adapt micropropagation protocols. The objective of this study was to test concentrations of Murashige & Skoog (MS) culture media and BAP (6-benzylaminopurine) and IBA (indolbutyric acid) plant growth regulators in the multiplication and in vitro rooting stages as part of the elaboration of a Chardonnay grapevine micropropagation protocol. In both experiments, multiplication and rooting, the experimental design was completely randomized, with a bifactorial scheme, testing culture media (MS and MS/2) and BAP concentrations (0; 5; 10 &#956;M) in the multiplication and of IBA (0; 0.5; 1.0; 1.5 &#956;M) in rooting. For multiplication, the best results for number of buds, leaves and shoots were obtained with 10 &#956;M BAP, and the concentrations of the culture media had no significant effect for most of the variables. For rooting, the MS media provided better results. In relation to IBA, the number and average root length were influenced by the concentration used, with 0.5 and 1.0 &#956;M providing the best results. Grapevine explants may be multiplied in MS/2 media supplemented with 10 &#956;M BAP and subsequently rooted in MS media with 0.5 &#956;M IBA.

Palabras-Clave: | Auxin; Cytokinin; MICROPROPAGATION; TISSUE CULTURE.
Thesagro: | VITIS VINIFERA.
Categoría del tema: | F01 Cultivo

URL:

http://pccm.ufla.br/index.php/plantcellculturemicropropagation/article/view/136
http://www.ainfo.inia.uy/digital/bitstream/item/15384/1/Nascimento-et-al.-2019.-In-vitro-multiplication-Plant-Cell.pdf

Registro original: | INIA Las Brujas (LB)
Ejemplares: | LB - (102547-1) - PXI - AP - DD - PP/PCCM/2019 -  -  - DIS