Fecha/hora: 13/06/2024 04:57:19

Biblioteca(s): 	INIA Las Brujas.	
Tipo de la producción científica: 	Artículos en Revistas Indexadas Internacionales	
Circulación/Nivel: 	Internacional - --	
Fecha corriente: 	03/02/2020	
Fecha de la última actualización: 	20/07/2022	
Autoria: 	MONTENEGRO, M.C.; PERAZA, P.; BALEMIAN, N.; CARBALLO, C.; BARLOCCO, N.; BARRIOS, P.G.; MERNIES, B.; SAADOUN, A.; CASTRO, G.; GUIMARÃES, S.F.; LLAMBÍ, S.	
Afiliación: 	M. C. MONTENEGRO, Departamento de Genética y Mejora Animal, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay; PABLO PERAZA DOS SANTOS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; N. BALEMIAN, Departamento de Genética y Mejora Animal, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay; C. CARBALLO, Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay; N. BARLOCCO, Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay; P. G. BARRIOS, Departamento de Biometría, Estadística y Cómputo, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay; B. MERNIES, Departamento de Genética y Mejora Animal, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay; A. SAADOUN, Sección Fisiología y Nutrición, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay; G. CASTRO, Departamento Animales de Granja, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay; S.F. GUIMARÃES, Departamento de Zootecnia, Universidade Federal de Viçosa, Minas Gerais, Brasil; S. LLAMBÍ, Departamento de Genética y Mejora Animal, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay.	
Título: 	Gene expression analysis by RNA-sequencing of Longissimus dorsi muscle of pigs fed diets with differing lipid contents.	
Año de publicación: 	2019	
Fuente/Imprenta: 	Genetics and Molecular Research, 2019, volume 18, number 4: GMR18307. OPEN ACCESS. Doi: https://doi.org/10.4238/gmr18307	
ISSN: 	1676-5680	
DOI: 	10.4238/gmr18307	
Idioma: 	Inglés	
Notas: 	Article history: Received April 05, 2019 / Accepted November 22, 2019 / Published December 31, 2019.
Corresponding Author: M.C. Montenegro - mariadc.montenegro@gmail.com	
Contenido: 	ABSTRACT.
We examined the effect of lipid content in the diet on the transcriptome of the Longissimus dorsi muscle in pigs. Our objective was to examine changes at the molecular level affecting economically relevant meat quality characteristics such as intramuscular fat deposition and fatty acid profile. The treatments consisted of isoproteic and isoenergetic diets with differing lipid contents due to addition of rice bran. The control diet (T0) was a normal basic diet and the test diet (T15) had 15% rice bran. The final lipid content (ether extract) in the diets was 3.4 and 4.8% in T0 and  T15, respectively. Three male piglets of the Uruguayan creole breed Pampa Rocha were used per treatment, which lasted from weaning at 42 days until 77 days of age. The animals were reared in confinement on deep bedding and were slaughtered at the end of the experiment, when muscle samples were collected. Intramuscular fat content (IMF) and fatty acid composition were analyzed to determine if diets had a phenotypic effect. Gene expression analysis was performed with RNA-seq methodology to carrying out a functional analysis of genes with differential expression between  treatments. The added fat to the diet did not affect IMF or fatty acid composition. However, we identified 359 genes with differential expression between treatments.
These genes participate in various metabolic pathways, some of them affecting meat quality. The most relevant genes identified in this regard were PDK4 (up-regulated with T15), which is associated with energy metabolism, FOS, ATF3, MYOD1 and MAFF (all downregulated with T15), which are associated with skeletal muscle growth, and TNC (up-regulated with T15), which is associated with extracellular matrix-receptor interactions. This study of the skeletal muscle transcriptome in pigs can help understand the genetic basis of how diet affects important production traits.	
Palabras-Clave: 	Candidate genes; Meat quality; Nutrigenomics; Skeletal muscle; Transcriptomic.	
Categoría del tema: 	L10 Genética y mejoramiento animal	
URL: 	https://www.geneticsmr.com/sites/default/files/articles/year2019/vol18-4/pdf/gmr18307_-_gene-expression-analysis-rna-sequencing-longissimus-dorsi-muscle-pigs-fed-diets-differing.pdf	
Registro original: 	INIA Las Brujas (LB)	
Ejemplares: 	LB - (102149-1) - PXI - AP - DD - PP/GMR/2019 -  -  - DIS