Fecha/hora: 16/06/2024 04:09:45

Biblioteca(s): | INIA Las Brujas.
Fecha corriente: | 24/03/2023
Fecha de la última actualización: | 27/04/2023
Autoria: | CORONA, M.; BRANCHICCELA, B.; ALBURAKI, M.; PALMER-YOUNG , E.C.; MADELLA , S.; CHEN, Y.; EVANS, J.D.
Afiliación: | MIGUEL CORONA, Bee Research Laboratory, United States Department of Agriculture, Beltsville, MD, United States; MARIA BELEN BRANCHICCELA CORREA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MOHAMED ALBURAKI, Bee Research Laboratory, United States Department of Agriculture, Beltsville, MD, United States; EVAN C. PALMER-YOUNG, Bee Research Laboratory, United States Department of Agriculture, Beltsville, MD, United States; SHAYNE MADELLA, Bee Research Laboratory, United States Department of Agriculture, Beltsville, MD, United States; YANPING CHEN, Bee Research Laboratory, United States Department of Agriculture, Beltsville, MD, United States; JAY D. EVANS, Bee Research Laboratory, United States Department of Agriculture, Beltsville, MD, United States.
Título: | Decoupling the effects of nutrition, age, and behavioral caste on honey bee physiology, immunity, and colony health.
Complemento del título: | Original Research article.
Año de publicación: | 2023
Fuente/Imprenta: | Frontiers in Physiology, 2023, Volume 14, article 1149840. OPEN ACCESS. doi: https://doi.org/10.3389/fphys.2023.1149840
ISSN: | 1664-042X (Online)
DOI: | 10.3389/fphys.2023.1149840
Idioma: | Inglés
Notas: | Article history: Received 23 January 2023; Accepted 22 February 2023; Published 13 March 2023. -- Correspondence author: Miguel Corona, Email:Miguel.Corona@usda.gov --  Edited by: Zhiguo Li, Fujian Agriculture and Forestry University, China. -- Reviewed by: Chunsheng Hou, Institute of Bast Fiber Crops (CAAS), China, Petar Hristov, Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, Bulgaria. -- This article is part of the Research Topic Biotic and Abiotic Stresses on Honeybee Physiology and and Colony Health (https://www.frontiersin.org/research-topics/41726/biotic-and-abiotic-stresses-on-honeybee-physiology-and-colony-health#articles ). -- LICENSE: This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). (http://creativecommons.org/licenses/by/4.0/ )
Contenido: | Nutritional stress, especially a dearth of pollen, has been linked to honey bee colony losses. Colony-level experiments are critical for understanding the mechanisms by which nutritional stress affects individual honey bee physiology and pushes honey bee colonies to collapse. In this study, we investigated the impact of pollen restriction on key markers of honey bee physiology, main elements of the immune system, and predominant honey bee viruses. To achieve this objective, we uncoupled the effects of behavior, age, and nutritional conditions using a new colony establishment technique designed to control size, demography, and genetic background. Our results showed that the expression of storage proteins, including vitellogenin (vg) and royal jelly major protein 1 (mrjp1), were significantly associated with nursing, pollen ingestion, and older age. On the other hand, genes involved in hormonal regulation including insulin-like peptides (ilp1 and ilp2) and methyl farnesoate epoxidase (mfe), exhibited higher expression levels in young foragers from colonies not experiencing pollen restriction. In contrast, pollen restriction induced higher levels of insulin-like peptides in old nurses. On the other hand, we found a strong effect of behavior on the expression of all immune genes, with higher expression levels in foragers. In contrast, the effects of nutrition and age were significant only the expression of the regulatory gene dorsal. We also found multiple interactions of the experimental variables on viral titers, including higher Deformed wing virus (DWV) titers associated with foraging and age-related decline. In addition, nutrition significantly affected DWV titers in young nurses, with higher titers induced by pollen ingestion. In contrast, higher levels of Black queen cell virus (BQCV) were associated with pollen restriction. Finally, correlation, PCA, and NMDS analyses proved that behavior had had the strongest effect on gene expression and viral titers, followed by age and nutrition. These analyses also support multiple interactions among genes and virus analyzed, including negative correlations between the expression of genes encoding storage proteins associated with pollen ingestion and nursing (vg and mrjp1) with the expression of immune genes and DWV titers. Our results provide new insights into the proximal mechanisms by which nutritional stress is associated with changes in honey bee physiology, immunity, and viral titers. Copyright © 2023 Corona, Branchiccela, Alburaki, Palmer-Young, Madella, Chen and Evans.
Palabras-Clave: | Dorsal; DWV; Immune genes; Insulin; MFE; MRJP1; Vitellogenin.
Thesagro: | APICULTURA; APIS MELLIFERA.
Categoría del tema: | A50 Investigación agraria
URL: | https://www.frontiersin.org/articles/10.3389/fphys.2023.1149840/pdf
Registro original: | INIA Las Brujas (LB)
Ejemplares: | LB - (103344-1) - PXI - AP - DD - Frontiers in Physiology/2023 -  -  - DIS