Fecha/hora: 16/06/2024 10:04:13

Biblioteca(s): 	INIA Las Brujas.	
Fecha corriente: 	03/12/2019	
Fecha de la última actualización: 	01/06/2020	
Autoria: 	LADO, J.; ZACARÍAS, J.; RODRIGO, M.J.; ZACARÍAS, L.	
Afiliación: 	JOANNA LADO LINDNER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JAIME ZACARIAS, Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas (IATA-CSIC), Spain; MARÍA JESÚS RODRIGO, Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas (IATA-CSIC), Spain; LORENZO ZACARÍAS, Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas (IATA-CSIC), Spain.	
Título: 	Visualization of carotenoid-storage structures in fruits by transmission electron microscopy.	
Año de publicación: 	2020	
Fuente/Imprenta: 	In: Rodríguez-Concepción M., Welsch R. (eds). Plant and Food Carotenoids. Methods in Molecular Biology, 2020, vol 2083. Pages 235-244. DOI: https://doi.org/10.1007/978-1-4939-9952-1_18	
ISBN: 	978-1-4939-9951-4 (print) / 978-1-4939-9952-1 (e-book)	
ISSN: 	1064-3745 (print) / 1940-6029 (electronic)	
DOI: 	10.1007/978-1-4939-9952-1_18	
Idioma: 	Inglés	
Notas: 	Article history: First Online: 20 November 2019.	
Contenido: 	ABSTRACT.
Plastids are cell organelles that, beside other functions, have the capability to store carotenoids in specialized structures, which may vary among the different plant species, tissues or according to the carotenoid complement. Fruits are an important source of carotenoids, and during ripening, chloroplasts differentiate into chromoplasts that are able to accumulate large amounts of carotenoids, rendering then the characteristic fruit coloration. Whereas lycopene or &#946;-carotene may accumulate as crystal in the chromoplasts of some fruit, other xanthophyll-accumulating fruits differentiate plastoglobuli as a preferred system to enhance carotenoids stability and storage. Visualization of plastid ultrastructure and their transformation during ripening or in fruit of contrasting coloration are fundamental objectives within carotenoids research in fruits. Therefore, in this chapter, we describe a protocol for the visualization and analysis of plastid ultrastructure by transmission electron microscopy (TEM), specially designed and adapted to fruit tissues.
© Springer Science+Business Media	
Palabras-Clave: 	CAROTENOIDS; Chloroplast; Chromoplast; CITRUS FRUITS; Plastids; Plastoglobuli; Transmission electron microscopy.	
Thesagro: 	CITRUS.	
Categoría del tema: 	--	
Registro original: 	INIA Las Brujas (LB)	
Ejemplares: 	LB - (102015-1) - PXI - PL - DD - Methods in Molecular Biology/2020 -  -  - DIS