Fecha/hora: 03/06/2024 03:55:45

Biblioteca(s): 	INIA Las Brujas.	
Tipo de la producción científica: 	Artículos en Revistas Indexadas Internacionales	
Circulación/Nivel: 	A - 1	
Fecha corriente: 	21/02/2014	
Fecha de la última actualización: 	28/10/2019	
Autoria: 	ORSOMANDO, G.; LORENZI, M.; RAFFAELLI, N.; DALLA RIZZA, M.; MEZZETTI, B.; RUGGIERI, S.	
Afiliación: 	GIUSEPPE ORSOMANDO, Dipto. Biotecnol. Agrarie Ambientali, Facoltà di Agraria, University of Ancona, Italy; MARIA LORENZI, Dipto. Biotecnol. Agrarie Ambientali, Facoltà di Agraria, University of Ancona, Italy; NADIA RAFFAELLI, Istituto di Biochimica, Facolta di Medicina e Chirurgia, University of Ancona, Italy; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Dipto. Biotecnol. Agrarie Ambientali, Facoltà di Agraria, University of Ancona, Italy; BRUNO MEZZETTI, Dipto. Biotecnol. Agrarie Ambientali, Facoltà di Agraria, University of Ancona, Italy; SILVERIO RUGGIERI, Dipto. Biotecnol. Agrarie Ambientali, Facoltà di Agraria, University of Ancona, Italy; Dipto. Biotecnol. Agrarie Ambientali, Università di Ancona, Italy.	
Título: 	Phytotoxic protein PcF, purification, characterization, and cDNA sequencing of a novel hydroxyproline-containing factor secreted by the strawberry pathogen phytophthora cactorum.	
Año de publicación: 	2001	
Fuente/Imprenta: 	Journal of Biological Chemistry, 15 June 2001, Volume 276, Issue 24, pp. 21578-21584.	
ISSN: 	0021-9258 (print)	
DOI: 	10.1074/jbc.M101377200	
Idioma: 	Inglés	
Notas: 	Article history: Received for publication, February 13, 2001 / In revised form, March 21, 2001 / Published, JBC Papers in Press, March 21, 2001.
This work was supported by a grant from the Regione Marche 5b.
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
This work is dedicated to the memory of Professor Pasquale Rosati.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number(s) AF354650.	
Contenido: 	ABSTRACT.
A novel protein factor, named PcF, has been isolated from the culture filtrate of Phytophthora cactorum strain P381 using a highly sensitive leaf necrosis bioassay with tomato seedlings. Isolated PcF protein alone induced leaf necrosis on its host strawberry plant. The primary structure and cDNA sequence of this novel phytotoxic protein was determined, and BLAST searches of Swiss-Prot, EMBL, and GenBank?/EBI data banks showed that PcF shared no significant homology with other known sequences. The 52-residue PcF protein, which contains a 4-hydroxyproline residue along with three S-S bridges, exhibits a high content of acidic sidechains, accounting for its isoelectric point of 4.4. The molecular mass of isolated PcF is 5,622 ± 0.5 Da as determined by mass spectrometry and matches that calculated from the deduced amino acid sequence with cDNA sequencing. The cDNA sequence indicates that PcF is first produced as a larger precursor, comprising an additional N-terminal, 21-residue secretory signal peptide. Maturation of this protein involves the hydroxylation of proline 49, a feature that is unique among other known secreted fungal phytopathogenic proteins.	
Palabras-Clave: 	BIODIVERSITY; Fragaria x ananassa; Molecular Sequence Data.	
Categoría del tema: 	F30 Genética vegetal y fitomejoramiento	
URL: 	http://www.jbc.org/content/276/24/21578.full.pdf	
Registro original: 	INIA Las Brujas (LB)	
Ejemplares: 	LB - (12821-1) - PXI - AP - DD - PP/Jr. Biological Chemistry/2001 -  -  - DIS