Fecha/hora: 16/06/2024 09:04:11

Biblioteca(s): | INIA Tacuarembó; INIA Treinta y Tres.
Fecha corriente: | 07/06/2019
Fecha de la última actualización: | 18/11/2021
Autoria: | IRISARRI, P.; CARDOZO, G.; TARTAGLIA, C.; REYNO, R.; GUTIÉRREZ, P.; LATTANZI, F.; REBUFFO, M.; MONZA, J.
Afiliación: | PILAR IRISARRI, Laboratorio de Microbiología, Departamento de Biología Vegetal, Facultad de Agronomía, UDELAR, URUGUAY; GERONIMO AGUSTIN CARDOZO CABANELAS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CAROLINA TARTAGLIA, Laboratorio de Bioquimica, Departamento de Biología Vegetal, Facultad de Agronomía, UDELAR, Uruguay.; RAFAEL ALEJANDRO REYNO PODESTA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PAMELA GUTIÉRREZ, Laboratorio de Bioquímica, Departamento de Biología Vegetal, Facultad de Agronomía, UDELAR, URUGUAY.; FERNANDO A. LATTANZI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MONICA IRENE REBUFFO GFELLER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JORGE MONZA, Laboratorio de Bioquímica, Departamento de Biología Vegetal, Facultad de Agronomía, UDELAR, URUGUAY.
Título: | Selection of competitive and efficient rhizobia strains for white clover.
Año de publicación: | 2019
Fuente/Imprenta: | Frontiers in Microbiology, 23 April 2019, v. 10, art. 768. DOI:  https://doi.org/10.3389/fmicb.2019.00768
Páginas: | 11 p.
DOI: | 10.3389/fmicb.2019.00768
Idioma: | Inglés
Notas: | Article history: Received 28 September 2018 // Accepted 26 March 2019 // Published: 23 April 2019.  Open Access Journal. Acknowledgments: We are grateful to Verónica Berriel for the N abundance determination and to Carlos Rossi for providing white clover seeds.
Contenido: | The practice of inoculating forage legumes with rhizobia strains is widespread. It is assumed that the inoculated strain determines the performance of the symbiosis and nitrogen fixation rates. However, native-naturalized strains can be competitive, and actual nodule occupancy is often scarcely investigated. In consequence, failures in establishment, and low productivity attributed to poor performance of the inoculant may merely reflect the absence of the inoculated strain in the nodules. This study lays out a strategy followed for selecting a Rhizobium leguminosarum sv. trifolii strain for white clover (Trifolium repens) with competitive nodule occupancy. First, the competitiveness of native-naturalized rhizobia strains selected for their efficiency to fix N2 in clover and tagged with gusA was evaluated in controlled conditions with different soils. Second, three of these experimental strains with superior nodule occupancy plus the currently recommended commercial inoculant, an introduced strain, were tested in the field in 2 years and at two sites. Plant establishment, herbage productivity, fixation of atmospheric N2 (15N natural abundance), and nodule occupancy (ERIC-PCR genomic fingerprinting) were measured. In both years and sites, nodule occupancy of the nativenaturalized experimental strains was either higher or similar to that of the commercial inoculant in both primary and secondary roots. The difference was even greater in stolon roots nodules, where nodule occupancy of the native-naturalized experimental strains was at least five times greater. The amount of N fixed per unit plant mass was consistently higher with native-naturalized experimental strains, although the proportion of N derived from atmospheric fixation was similar for all strains. Plant establishment and herbage production, as well as clover contribution in oversown native grasslands, were either similar or higher in white clover inoculated with the native-naturalized experimental strains. These results support the use of our implemented strategy for developing a competitive inoculant from native-naturalized strains.
Palabras-Clave: | BIOLOGICAL NITROGEN FIXATION; INOCULANT; NATIVE-NATURALIZED RHIZOBIA STRAINS; NODULE OCCUPANCY; R. LEGUMINOSARUM SV. TRIFOLII; WHITE CLOVER.
Thesagro: | FIJACION BIOLOGICA DEL NITRÓGENO; NODULACION; TREBOL BLANCO; TRIFOLIUM REPENS.
Categoría del tema: | F60 Fisiología y bioquímica de la planta
URL: | http://www.ainfo.inia.uy/digital/bitstream/item/12797/1/Cardozo-Frontier-Micro-2019.pdf
Registro original: | INIA Tacuarembó (TBO)

Ejemplares:

TBO - (102974-1) - INI - AP - DD - PP/FRONTIERS IN MICROBIOLOY/2019/768 -  -  - DIS
TT - (102773-1) - PXI - AP - DD - PP/FRONTIERS IN MICROBIOLOY/2019/768 -  -  - DIS