Fecha/hora: 16/06/2024 06:40:22

Biblioteca(s): 	INIA Tacuarembó.	
Fecha corriente: 	21/02/2014	
Fecha de la última actualización: 	30/09/2019	
Autoria: 	DO CANTO, J.; STUDER, B.; FREI, U.; LÜBBERSTEDT, T.	
Afiliación: 	JAVIER DO CANTO FAGUNDEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; BRUNO STUDER, Molecular Plant Breeding, Institute of Agricultural Sciences, ETH Zurich, Universitaetstrasse 2, 8092 Zurich, Switzerland.; URSULA FREI, Department of Agronomy, Iowa State University, 1204 Agronomy Hall, Ames, IA 50011?1010, USA.; THOMAS LÜBBERSTEDT, Department of Agronomy, Iowa State University, 1204 Agronomy Hall, Ames, IA 50011?1010, USA.	
Título: 	Fine mapping a self-fertility locus in perennial ryegrass.	
Año de publicación: 	2018	
Fuente/Imprenta: 	Theoretical and Applied Genetics, v. 131, no. 4, p. 817-827, 2018.	
DOI: 	10.1007/s00122-017-3038-6	
Idioma: 	Inglés	
Notas: 	Article history: Received: 2 August 2017 //Accepted: 11 December 2017.	
Contenido: 	Key message A self-fertility locus was fine mapped to a 1.6 cM region on linkage group 5 in a perennial ryegrass population. This locus was the main determinant of pollen self-compatibility. Abstract In grasses, self-incompatibility (SI) is characterized by a two-loci gametophytic (S and Z) mechanism acting together in the recognition and inhibition of self-pollen. Mutations affecting the expression of SI have been reported in a few grass species. In perennial ryegrass (Lolium perenne L.), a mutation independent from S and Z, and mapping on linkage group 5 (LG 5), was previously reported to produce self-fertile plants. Here, we describe fine mapping of the self-fertility (SF) gene in a perennial ryegrass population and determine whether there is any effect of other genomic regions on the pollen compatibility. The phenotypic segregation of SF showed a bimodal distribution with one mean at 49% pollen compatibility and the other at 91%. Marker-trait association analysis showed that only markers on LG 5 were significantly associated with the trait. A single gene model explained 82% of the observed variability and no effects of the other regions were detected. Using segregation and linkage analysis, the SF locus was located to a 1.6 cM region on LG 5. The flanking marker sequences were aligned to rice and Brachypodium distachyon reference genomes to estimate the physical distance. We provide markers tightly linked to SF that can be used for introgression of this trait into advanced breeding germplasm. Moreover, our results represent a further step towards the identification of the SF gene in LG 5.	
Thesagro: 	GRAMINEAS; LOLIUM PERENNE.	
Categoría del tema: 	A50 Investigación agraria	
Registro original: 	INIA Tacuarembó (TBO)	
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