Fecha/hora: 28/05/2024 15:12:06

Biblioteca(s): 	INIA La Estanzuela.	
Tipo de la producción científica: 	Artículos en Revistas Indexadas Internacionales	
Circulación/Nivel: 	Internacional - --	
Fecha corriente: 	11/07/2022	
Fecha de la última actualización: 	02/12/2022	
Autoria: 	GARCÍA-ROCHE, M.; TALMÓN, D.; CAÑIBE, G.; ASTESSIANO, A.L.; MENDOZA, A.; QUIJANO, C.; CASSINA, A.; CARRIQUIRY, M.	
Afiliación: 	MERCEDES GARCÍA-ROCHE V, Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, 12900, Montevideo, Uruguay.; DANIEL TALMÓN, Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, 12900, Montevideo, Uruguay.; GUILLERMO CAÑIBE, Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, 12900, Montevideo, Uruguay.; ANA LAURA ASTESSIANO, Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, 12900, Montevideo, Uruguay.; ALEJANDRO FRANCISCO MENDOZA AGUIAR, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CELIA QUIJANO, Centro de Investigaciones Biomédicas (CEINBIO) and Departamento de Bioquímica, Facultad de Medicina, Universidad de la República, 11900, Montevideo, Uruguay.; ADRIANA CASSINA, Centro de Investigaciones Biomédicas (CEINBIO) and Departamento de Bioquímica, Facultad de Medicina, Universidad de la República, 11900, Montevideo, Uruguay.; MARIANA CARRIQUIRY, Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, 12900, Montevideo, Uruguay.	
Título: 	Differential hepatic mitochondrial function and gluconeogenic gene expression in 2 Holstein strains in a pasture-based system.	
Año de publicación: 	2022	
Fuente/Imprenta: 	Journal of Dairy Science, July 2022, Volume 105, Issue 7, Pages 5723-5737. OPEN ACCESS. doi: https://doi.org/10.3168/jds.2021-21358	
ISSN: 	0022-0302	
DOI: 	10.3168/jds.2021-21358	
Idioma: 	Inglés	
Notas: 	Article history: Received 30 September 2021, Accepted 17 March 2022, Available online 20 May 2022, Version of Record 22 June 2022. -- Corresponding author:  García-Roche, M.; Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay; email:mercedesg@fagro.edu.uy -- LICENSE: This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). --  Funding: The authors thank all the staff of the Dairy Unit of the Experimental Station ?La Estanzuela? (Semillero, Uruguay) for their support in animal handling, as well as Ezequiel Jorge-Smeding (Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay) and Irene Benoit (Centro de Investigaciones Biomédicas and Departamento de Bioquímica, Facultad de Medicina, Universidad de la República, Montevideo) for support in the analysis of blood metabolites. MGR conceived and designed laboratory experiments, performed field and laboratory experiments, analyzed the results, and wrote the original draft and revised versions of the manuscript. MC conceptualized and designed field experiment, conceived and designed laboratory experiments, supervised field and laboratory experiments and results analyses, obtained funding and administrated the project, and reviewed and edited the manuscript. CQ conceived, designed, and supervised laboratory experiments, and reviewed and edited the manuscript. AC conceived, designed, and supervised laboratory experiments. AM conceptualized and designed the field experiment and obtained funding. DT, ALA, and GC performed field and laboratory experiments. All authors reviewed the manuscript and approved the final version of the manuscript. M. García-Roche was supported by Comisión Académica de Posgrados (Montevideo, Uruguay) fellowship BDDX_2018_1#49004502. D. Talmón was supported by Agencia Nacional de Investigación e Innovación (ANII) fellowship POS_NAC_2017_1_141266. A. Cassina and C. Quijano were partially funded by grants of the Espacio Interdisciplinario-Centros, Universidad de la República (UdelaR; Montevideo, Uruguay) 2015. A. Cassina was also supported by the grant Comisión Sectorial de Investigación Científica (CSIC) grupos I+D 2014 (767). The project was funded by CSIC of the UdelaR CSIC I+D 2018 ID 103 to M. Carriquiry and C. Quijano, as well as by ANII INNOVAGRO 2018: FSA_1_2018_1_152220 to M. Carriquiry and A. Cassina. A. Mendoza received funding from the project PL_21_0_00 of the Instituto Nacional de Investigación Agropecuaria (Semillero, Uruguay). The authors have not stated any conflicts of interest.	
Contenido: 	ABSTRACT:
The objective of this study was to assess hepatic ATP synthesis in Holstein cows of North American and New Zealand origins and the gluconeogenic pathway, one of the pathways with the highest ATP demands in the ruminant liver. Autumn-calving Holstein cows of New Zealand and North American origins were managed in a pasture-based system with supplementation of concentrate that represented approximately 33% of the predicted dry matter intake during 2017, 2018, and 2019, and hepatic biopsies were taken during mid-lactation at 174 ± 23 days in milk. Cows of both strains produced similar levels of solids-corrected milk, and no differences in body condition score were found. Plasma glucose concentrations were higher for cows of New Zealand versus North American origin. Hepatic mitochondrial function evaluated measuring oxygen consumption rates showed that mitochondrial parameters related to ATP synthesis and maximum respiratory rate were increased for cows of New Zealand compared with North American origin. However, hepatic gene expression of pyruvate carboxylase, phosphoenolpyruvate carboxykinase, and pyruvate dehydrogenase kinase was increased in North American compared with New Zealand cows. These results altogether suggest an increased activity of the tricarboxylic cycle in New Zealand cows, leading to increased ATP synthesis, whereas North American cows pull tricarboxylic cycle intermediates toward gluconeogenesis. The fact that this occurs during mid-lactation could account for the increased persistency of North American cows, especially in a pasture-based system. In addition, we observed an augmented mitochondrial density in New Zealand cows, which could be related to feed efficiency mechanisms. In sum, our results contribute to the elucidation of hepatic molecular mechanisms in dairy cows in production systems with higher inclusion of pastures. © 2022 American Dairy Science Association.	
Palabras-Clave: 	Dairy cows; Gluconeogenesis; Grazing; Holstein-Friesian; Intermediary metabolismg.	
Thesagro: 	VACAS LECHERAS.	
Categoría del tema: 	--	
URL: 	http://www.ainfo.inia.uy/digital/bitstream/item/16559/1/J.-Dairy-Sci.-105p.57235737-Mendoza.pdf	
Registro original: 	INIA La Estanzuela (LE)	
Ejemplares: 	LE - (103590-1) - PXI - AP - DD - PP/JDS/2022 -  -  - DIS