Fecha/hora: 13/06/2024 09:07:19

Biblioteca(s): 	INIA Las Brujas.	
Fecha corriente: 	24/06/2019	
Fecha de la última actualización: 	16/03/2021	
Autoria: 	CASAL, A.; GARCÍA-ROCHE, M.; NAVAJAS, E.; CASSINA, A.; CARRIQUIRY, M	
Afiliación: 	A. CASAL, Departamento de Produccion Animal y Pasturas, Facultad de Agronomia, Universidad de la Republica, Paysandú, Uruguay.; M. GARCÍA-ROCHE, Departamento de Produccion Animal y Pasturas, Facultad de Agronomia, Universidad de la Republica, Montevideo, Uruguay; Center for Free Radical and Biomedical Research (CEINBIO); ELLY ANA NAVAJAS VALENTINI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; A. CASSINA, Center for Free Radical and Biomedical Research (CEINBIO) and Departamento de Bioquímica, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay; M. CARRIQUIRY, Departamento de Produccion Animal y Pasturas, Facultad de Agronomia, Universidad de la Republica, Montevideo, Uruguay.	
Título: 	Differential hepatic oxidative status in steers with divergent residual feed intake phenotype.	
Año de publicación: 	2020	
Fuente/Imprenta: 	Animal, 1 January 2020, Volume 14, Issue 1, Pages 78-85. Doi: https://doi.org/10.1017/S1751731119001332	
DOI: 	10.1017/S1751731119001332	
Idioma: 	Inglés	
Notas: 	Article history: Received 12 December 2018 // Accepted 6 May 2019 // Published online: 20 June 2019.
Supplementary materials.
Corresponding author: Casal, A.; Departamento de Produccion Animal y Pasturas, Facultad de Agronomia, Universidad de la Republica, Ruta 3 km 363, Paysandu, Uruguay; email:alcas@adinet.com.uy	
Contenido: 	ABSTRACT.
Oxidative stress occurs when oxidant production exceeds the antioxidant capacity to detoxify the reactive intermediates or to repair the resulting damage. Feed efficiency has been associated with mitochondrial function due to its impact on cell energy metabolism. However, mitochondria are also recognized as a major source of oxidants. The aim of this study was to determine lipid and protein oxidative stress markers, and gene and protein expression as well as activity of antioxidant enzymes in the liver of steers of divergent residual feed intake (RFI) phenotypes. Hereford steers (n = 111) were evaluated in post-weaning 70 days standard test for RFI. Eighteen steers exhibiting the greatest (n = 9; high-RFI) and the lowest (n = 9; low-RFI) RFI values were selected for this study. After the test, steers were managed together under grazing conditions until slaughter when they reached the slaughter body weight. At slaughter, hepatic samples were obtained, were snap-frozen in liquid nitrogen and stored at &#8722;80°C until analyses. Hepatic thiobarbituric acid reactive species and protein carbonyls were greater (P = 0.05) and hepatic 4-hydroxynonenal protein adducts tended (P = 0.10) to be greater for high- than low-RFI steers. Hepatic gene expression glutathione peroxidase 4, glutamate?cysteine ligase catalytic subunit and peroxiredoxin 5 mRNA was greater (P &#8804; 0.05) and glutathione peroxidase 3 mRNA tended (P = 0.10) to be greater in low- than high-RFI steers. Hepatic protein expression and enzyme activity of manganese superoxide dismutase and glutathione peroxidase enzyme activity tended (P &#8804; 0.10) to be greater for low- than high-RFI steers. High-efficiency steers (low-RFI) probably had better hepatic oxidative status which was strongly associated with greater antioxidant ability near to the oxidant production site and, therefore, reduced oxidative stress of the liver. Decreased hepatic oxidative stress would reduce maintenance requirements due to a lower protein and lipid turnover and better efficiency in the use of energy.
© The Animal Consortium 2019	
Palabras-Clave: 	ANTIOXIDANTS; ENERGY METABOLISM; FEED EFFICIENCY; FEEDING; LIVER; OXIDATIVE STRESS.	
Thesagro: 	ALIMENTACION ANIMAL.	
Categoría del tema: 	L02 Alimentación animal	
Registro original: 	INIA Las Brujas (LB)	
Ejemplares: 	LB - (101842-1) - PXI - AP - DD - PP/ANIMAL/2020 -  -  - DIS