Fecha/hora: 16/06/2024 05:32:39

Biblioteca(s): 	INIA Las Brujas.	
Fecha corriente: 	21/02/2014	
Fecha de la última actualización: 	05/02/2020	
Autoria: 	QUAGLIOTTO, L.; AZZIZ, G.; BAJSA, N.; VAZ, P.; PÉREZ, C.; DUCAMP, F.; CADENAZZI, M.; ALTIER, N.; ARIAS, A.	
Afiliación: 	LETICIA QUAGLIOTTO, Laboratorio de Ecología Microbiana, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay; GASTÓN AZZIZ, Laboratorio de Ecología Microbiana, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay; NATALIA BAJSA, Laboratorio de Ecología Microbiana, Instituto de Investigaciones Biológicas Clemente Estable, ¡Montevideo, Uruguay; Sección Bioquímica, Facultad de Ciencias, UdelaR, Montevideo, Uruguay; PATRICIA VAZ, Laboratorio de Ecología Microbiana, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay; CARLOS PÉREZ, Departamento de Protección Vegetal, Facultad de Agronomía, Universidad de la República, Paysandú, Uruguay; FERNANDO DUCAMPO, Departamento de Protección Vegetal, Facultad de Agronomía, Universidad de la República, Paysandú, Uruguay; MÓNICA CADENAZZI, Departamento de Protección Vegetal, Facultad de Agronomía, Universidad de la República, Paysandú, Uruguay; NORA ADRIANA ALTIER MANZINI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ALICIA ARIAS, Laboratorio de Ecología Microbiana, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.	
Título: 	Three native Pseudomonas fluorescens strains tested under growth chamber and field conditions as biocontrol agents against damping-off in alfalfa.	
Año de publicación: 	2009	
Fuente/Imprenta: 	Biological Control, October 2009, Volume 51, Issue 1, Pages 42-50. Doi: https://doi.org/10.1016/j.biocontrol.2009.05.006	
ISSN: 	1049-9644	
DOI: 	10.1016/j.biocontrol.2009.05.006	
Idioma: 	Inglés	
Notas: 	Article history: Received 1 September 2008 / Accepted 22 May 2009 / Available online 28 May 2009.
Corresponding author: Leticia Quagliotto - letty@iibce.edu.uy
Funding information: This work was financially supported by the LIA-INIA 028 Project, in the frame of the "Programa de Servicios Agropecuarios 1131-OC-UR (BID-MGAP-INIA)".	
Contenido: 	ABSTRACT.
Alfalfa (Medicago sativa) is one of the most important crops used in Uruguay for livestock feeding. Seedling diseases, particularly damping-off, are a critical factor which limits its establishment. Three native Pseudomonas fluorescens strains, UP61.2, UP143.8 and UP148.2, previously isolated from Lotus corniculatus, were evaluated to determine their efficacy as biological control agents for alfalfa seedling diseases in the field. Their compatibility with the alfalfa-Sinorhizobium meliloti symbiosis was also assessed. In growth chamber conditions seed inoculation with Pseudomonas strains did not affect different parameters of alfalfa-rhizobium symbiosis as shown by nodulation rate and shoot dry weight of plants. The presence of the commercial inoculant strains of S. meliloti did not impair colonization by the P. fluorescens and vice versa. In field trials the dynamics of rhizobial rhizospheric populations were not affected by the presence of P. fluorescens. Each P. fluorescens strain successfully colonized alfalfa roots at adequate densities for biocontrol activity. Results showed that P. fluorescens strains provided a 10-13% increase in the number of established plants relative to the control, an intermediate result compared to the fungicide treatment (24%). The alfalfa above-ground biomass was increased by 13% and 15-18% in the presence of the fungicide and P. fluorescens strains, respectively. Therefore, results from this study demonstrated that the three P. fluorescens strains provided effective control against soil-borne pathogens and suggest a potential use in the development of a commercial inoculant to be applied for the control of legume seedling diseases. 
© 2009 Elsevier Inc. All rights reserved.	
Palabras-Clave: 	Biological control agent; Damping-off; Field trials; Growth chamber conditions.	
Thesagro: 	CONTROL BIOLÓGICO; MEDICAGO SATIVA; PSEUDOMONAS FLUORESCENS.	
Categoría del tema: 	F01 Cultivo	
Registro original: 	INIA Las Brujas (LB)	
Ejemplares: 	LB - (12817-1) - PXI - AP - DD - PP/BIOLOGICAL CONTROL/2009 -  -  - DIS