Fecha/hora: 08/06/2024 17:37:57

Biblioteca(s): 	INIA Las Brujas.	
Fecha corriente: 	02/02/2022	
Fecha de la última actualización: 	02/02/2022	
Autoria: 	TORRES, D.; DELGADO-CERRONE, L.; LUNA, L.; RESQUÍN, F.; AGUILAR, A.V.; SEBENN, A.M.	
Afiliación: 	DIEGO GABRIEL TORRES DINI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LEONARDO DELGADO-CERRONE, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Av. Italia 3318, 11600 Montevideo, Uruguay.; LORENA LUNA, Centro Universitario de Tacuarembó (CUT), Ruta 5 Km 386, CEP 45000, Tacuarembó, TB, Uruguay; JOSE FERNANDO RESQUIN PEREZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANANDA VIRGINIA AGUIAR, Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA), Centro Nacional de Pesquisa de Florestas, Estrada da Ribeira,  Km 111 - Bairro Guaraituba, 83411-000, Colombo, PR, Brazil.; ALEXANDRE MAGNO SEBBENN, Instituto Florestal de São Paulo, CP 1322, São Paulo, SP, 01059-970, Brazil.	
Título: 	The traceability of Eucalyptus clones using molecular markers.	
Año de publicación: 	2021	
Fuente/Imprenta: 	Silvae Genetica, 2021, 70(1) 217-225. OPEN ACCESS. https://doi.org/10.2478/sg-2021-0019	
DOI: 	10.2478/sg-2021-0019	
Idioma: 	Inglés	
Notas: 	Article history: Published Online 25 Dec 2021.
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Corresponding author: Diego Torres-Dini, E-mail: diego.torres.dini@gmail.com	
Contenido: 	ABSTRACT.- The improvement of Eucalyptus clones plays a crucial role in modern silviculture. This study used a set of 17 microsatellite loci to analyze the genetic diversity and structure of 107 elite clones (80 E. grandis and 27 E. globulus). All clones were cultivated in Uruguay and were sourced from three different providers. Using the fingerprinting technique, an exclusive molecular profile was assigned for each clone, and the genotyping reaction showed differences between the two species. The cumulative probability of identifying two random individuals that share the same genotype (???? ) with all 17 loci, was estimated as low for E. grandis (1.18×10-15) and E. globulus (4.03×10-14). The combined PIsibs was (1.05×10-5) and (2.17×10-5) for E. grandis and E. globulus, respectively. A total of 180 alleles were detected for E. grandis and 100 for E. globulus. We found a high mean number of alleles per locus (10 for E. grandis and 6 for E. globulus), and the results for mean polymorphic information content ( PIC ) were (0.648) and (0.548), respectively. The observed heterozygosity ( Ho ) ranged from 0.216 to 0.838 (mean = 0.509) for E. grandis and 0 to 1 (mean = 0.566) for E. globulus. Two core sets of seven EST-SSR loci were identified for each species. These markers revealed unambiguous fragment amplification, providing a minimum number of SSRs for effective clonal identification. The genetic structure analysis suggests that the germplasm of the E. grandis population is structured in four clusters, while the E. globulus population consists of two clusters.
© 2020 Diego Torres-Dini et al., published by Sciendo	
Palabras-Clave: 	Clone; Clone certification; Genotyping; Identity; Multiplex; Nurseries; Traceability.	
Categoría del tema: 	K01 Ciencias forestales - Aspectos generales	
URL: 	https://sciendo.com/pdf/10.2478/sg-2021-0019	
Registro original: 	INIA Las Brujas (LB)	
Ejemplares: 	LB - (102988-1) - PXI - AP - DD - PP/Silvae Genetica/2021 -  -  - DIS