Fecha/hora: 09/06/2024 18:26:36

Biblioteca(s): 	INIA Las Brujas.	
Tipo de la producción científica: 	Artículos en Revistas Indexadas Internacionales	
Circulación/Nivel: 	A - 1	
Fecha corriente: 	11/09/2014	
Fecha de la última actualización: 	24/06/2021	
Autoria: 	SOUZA, A.L.A.; DÍAZ DELLAVALLE, P.; CABRERA, A.; LARRAÑAGA, P.; DALLA RIZZA, M.; DE-SIMONE, S.G.	
Afiliación: 	PAOLA ALEXANDRA DIAZ DELLAVALLE, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; ANDREA CECILIA CABRERA BARRIOS, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MARCO DALLA RIZZA VILARO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay.	
Título: 	Antimicrobial activity of pleurocidin is retained in Plc-2, a C-terminal 12-amino acid fragment.	
Año de publicación: 	2013	
Fuente/Imprenta: 	Peptides, 2013, v.45, p.78-84.	
ISSN: 	0196-9781	
DOI: 	10.1016/j.peptides.2013.03.030	
Idioma: 	Inglés	
Contenido: 	ABSTRACT.

An analysis of a series of five peptides composed of various portions of the pleurocidin (Plc) sequence identified a l2-amino acid fragment from the C-terminus of Plc, designated Plc-2, as the smallest fragment that retained a antimicrobial activity comparable to that of the parent compound. MIC tests in vitro with low-ionic-strength medium showed that Plc-2 has potent activity against Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus but not against Enterococcus faecalis. The antifungal activity of the synthetic peptides against phytopathogenic fungi, such as Fusarium oxysporum, Colletotrichum sp., Aspergillus niger and Alternaria sp., also identified Plc-2 as a biologically active peptide. Microscopy studies of fluorescently stained fungi treated with Plc-2 demonstrated that cytoplasmic and nuclear membranes were compromised in all strains of phytopathogenic fungi tested. Together, these results identify Plc-2 as a potential antimicrobial agent with similar properties to its parent compound, pleurocidin. In addition, it demonstrated that the KHVGKAALTHYL residues are critical for the antimicrobial activity described for pleurocidin. 
© 2013 Elsevier Inc. All rights reserved.	
Thesagro: 	ALTERNARIA; HONGOS; PÉPTIDOS.	
Categoría del tema: 	H01 Protección de plantas - Aspectos generales	
Registro original: 	INIA Las Brujas (LB)	
Ejemplares: 	LB - (100048-1) - PXI - AP - DD - PP/PEPTIDES/2013 -  -  - DIS