Fecha/hora: 11/06/2024 20:25:16

Biblioteca(s): | INIA Las Brujas.
Fecha corriente: | 01/10/2019
Fecha de la última actualización: | 01/10/2019
Autoria: | CASTILLO, A.; CARRO, B. L.; DALLA RIZZA, M.; REYNO, R.
Afiliación: | ALICIA MARIA CASTILLO SALLE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; B. LÓPEZ CARRO, Flow Cytometry and Cell Sorting Core, Institute of Biological Research Clemente Estable,	Montevideo, Uruguay.; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RAFAEL ALEJANDRO REYNO PODESTA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.
Título: | Use of in vitro methods to induce autotetraploids in the native forage legume Trifolium polymorphum.
Complemento del título: | Conference Paper.
Año de publicación: | 2018
Fuente/Imprenta: | Acta Horticulturae, 15 November 2018, N° 1224, p. 73-80.
Série: | (Acta Horticulturae; 1224)
ISBN: | 978-94-62612-20-4
ISSN: | 0567-7572 (print) / 2406-6168 (electronic)
DOI: | 10.17660/ActaHortic.2018.1224.11
Idioma: | Inglés
Notas: | In: Acta Hortic. 1224. VII International Symposium on Production and Establishment of Micropropagated Plants. Editors: R. Paiva, M.V. Reis, D.P.C. Silva.

Contenido:

ABSTRACT.
The manipulation of ploidy is a helpful tool in improving crops and grasses. Trifolium polymorphum (Poir.) (Leguminosae) is a native perennial stoloniferous winter species that combines different types of reproductive systems. This biological advantage makes T. polymorphum a remarkable issue of research. T. polymorphum
belongs to the Campos region and has evolved into adapted ecotypes through long exposure to environmental stresses, grazing and by competing with the native C3 and C4 grasses which have dominated the Campos vegetation over a long period of time. Eurasiatic Trifolium species lack persistence in the temperate to subtropical
environment found in the Campos region and have to rely on their reseeding capacity to persist. T. polymorphum is currently being studied for its ability to adapt to
Uruguayan conditions. Despite this significant advantage in survival, the major constraint of T. polymorphum is a poor biomass production and low seedling vigor
that represents a limitation for its use as a forage legume. The objective of this work was to evaluate different in vitro methods to induce polyploidy. We evaluated two
antimitotic agents in different concentrations and time exposures: colchicine and oryzalin in different kinds of explant, and the effect of colchicine added in the culture
medium. Colchicine was more effective in polyploidy induction. In seedlings 0.3% of colchicine for 24 h achieved 11% of tetraploids, for in vitro explants 0.2% of
colchicine reached to 13% of chromosome duplication, and 16% when colchicine (0.015%) was added in the culture medium. The numbers of chloroplasts in stomatal
guard cells was used as a quick assessment of the ploidy level and then confirmed by flow cytometry.
@ISHS, International Society for Horticultural Science.

Palabras-Clave: | BIOMASS; COLCHICINE; ORYZALIN; POLYPLOIDY.
Categoría del tema: | F30 Genética vegetal y fitomejoramiento
Registro original: | INIA Las Brujas (LB)
Ejemplares: | LB - (101964-1) - PXI - AP - DD - PP/ACTA HORTICULTURAE/2018/1224 -  -  - DIS