Fecha/hora: 15/06/2024 19:20:42

Biblioteca(s): 	INIA Tacuarembó.	
Fecha corriente: 	18/09/2014	
Fecha de la última actualización: 	26/11/2020	
Autoria: 	KHU, D.; REYNO, R.; BRUMMER, E.; MONTEROS, M.	
Afiliación: 	DONG-MAN KHU, Forage Improvement Division, The Samuel Roberts Noble Foundation, 2510 Sam Noble Pkwy., Ardmore, USA; RAFAEL ALEJANDRO REYNO PODESTA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; E. CHARLES BRUMMER, Forage Improvement Division, The Samuel Roberts Noble Foundation, 2510 Sam Noble Pkwy., Ardmore, USA; MARÍA J. MONTEROS, Forage Improvement Division, The Samuel Roberts Noble Foundation, 2510 Sam Noble Pkwy., Ardmore, USA.	
Título: 	Screening methods for aluminum tolerance in Alfalfa.	
Año de publicación: 	2012	
Fuente/Imprenta: 	Crop Science, 2012, v. 52, p. 161 - 167. DOI: https://doi.org/10.2135/cropsci2011.05.0256	
Volumen: 	52	
DOI: 	10.2135/cropsci2011.05.0256	
Idioma: 	Inglés	
Notas: 	Article history: Published online 4 Oct. 2011. Acknowledgments:This work was funded by the Samuel Roberts Noble Foundation. We thank Christy Motes, Kazuyo Ueda, and Will Chaney
for their assistance with the whole plant assay in media and Donald Wood, Jonathan Markham, and Wesley Dean for help with the soil assay. We appreciate the assistance from Cook Ly
in preparing the fi gures and discussions with Joe Bouton.	
Contenido: 	Reliable phenotypic assays are essential to identify genetic variation for factors limiting crop productivity. Aluminum in the soil is highly phytotoxic and is a major limitation to crop production. Current methods to evaluate Al tolerance in alfalfa (Medicago sativa L.) based on root characteristics are time consuming, labor intensive, and subject to microenvironmental variation. Our objective was to develop two rapid whole-plant assays, one in culture media and one in soil, to evaluate acid and Al tolerance in alfalfa. The culture-media method involved measuring root growth and architecture 2 wk after growing the plants in media at pH 7.0 without Al, pH 4.0 without Al, and pH 4.0 with Al. The greenhouse-based soil method is simpler, faster, and more efficient than previously used soil assays. The two assays produced similar results, consistently distinguishing between Al-sensitive and Al-tolerant alfalfa genotypes in accord with previously described assays. These whole-plant assay methods will enable the screening of breeding populations to improve Al tolerance and the dissection of the genetic determinants underlying variation in tolerance responses.	
Palabras-Clave: 	CROP PRODUCTION; CROP PRODUCTIVITY; MEDICAGO SATIVA L.	
Thesagro: 	ALFALFA.	
Categoría del tema: 	A50 Investigación agraria	
Registro original: 	INIA Tacuarembó (TBO)	
Ejemplares: 	TBO - (100082-1) - PXI - AP - DD - PP/CROP SCIENCE/2012 -  -  - DIS