Fecha/hora: 16/06/2024 00:55:02

Biblioteca(s): 	INIA La Estanzuela.	
Fecha corriente: 	15/01/2020	
Fecha de la última actualización: 	05/09/2022	
Autoria: 	CASTELLS, M.; BERTONI, ESTEFANY; CAFFARENA, D.; CASAUX, M.L.; SCHILD, C.; VICTORIA, M.; RIET-CORREA, F.; GIANNITTI, F.; PARREÑO, V.; COLINA, R.	
Afiliación: 	MATÍAS CASTELLS BAUER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Laboratorio de Virología Molecular, CENUR Litoral Norte, Centro Universitario de Salto, Universidad de la República, Rivera 1350, Salto, Uruguay.; ESTEFANY BERTONI, Laboratorio de Virología Molecular, CENUR Litoral Norte, Centro Universitario de Salto, Universidad de la República, Rivera 1350, Salto, Uruguay.; RUBEN DARÍO CAFFARENA LEDESMA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA LAURA CASAUX, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS SCHILD, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MATIAS VICTORIA, Laboratorio de Virología Molecular, CENUR Litoral Norte, Centro Universitario de Salto, Universidad de la República, Rivera 1350, Salto, Uruguay.; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; VIVIANA PARREÑO, Sección de Virus Gastroentéricos, Instituto de Virología, CICV y A, INTA Castelar, Nicolás Repetto S/N, Buenos Aires 1686, Argentina.; RODNEY COLINA, Laboratorio de Virología Molecular, CENUR Litoral Norte, Centro Universitario de Salto, Universidad de la República, Rivera 1350, Salto, Uruguay.	
Título: 	Bovine astrovirus surveillance in Uruguay reveals high detection rate of a novel mamastrovirus species.	
Año de publicación: 	2020	
Fuente/Imprenta: 	Viruses, Dec 27, Vol. 12 , n.1, 2020. OPEN ACCESS. Doi: https://doi.org/10.3390/v12010032	
DOI: 	10.3390/v12010032	
Idioma: 	Inglés	
Notas: 	Article history: Received: 26 October 2019 / Revised: 15 November 2019 / Accepted: 15 November 2019 / Published: 27 December 2019.  Supplementary Materials: The following are available online at http://www.mdpi.com/1999-4915/12/1/32/s1.Figure S1: Maximum-likelihood tree constructed with complete capsid nucleotide sequences of MAstVs, Figure S2: Maximum-likelihood tree constructed with partial capsid amino acid sequences, Table S1: Partial polymerase sequences used for the phylogenetic analysis, and Table S2: Complete capsid sequences used for the MAstV
species classification.Author Contributions: Conceptualization, M.C. and R.C.; methodology, M.C., E.B., R.D.C., M.L.C. and C.S.;resources, M.C., F.R.-C., F.G. and R.C.; writing?original draft preparation, M.C.; writing?review and editing,M.C., E.B., R.D.C., M.L.C., C.S., M.V., F.R.-C., F.G., V.P. and R.C.; funding acquisition, M.C. and R.C. All authors have read and agreed to the published version of the manuscript. Funding: This research was funded by ?Comisión Sectorial de Investigación Científica? (CSIC), grant number ini2017_158 and ?Instituto Nacional de Investigación Agropecuaria? (INIA), grant number PL_015 N-15156. Acknowledgments: M.C. acknowledges support from the ?Agencia Nacional de Investigación e Innovación? (ANII) through a PhD scholarship.	
Contenido: 	Abstract:
Viral infections affecting cattle lead to economic losses to the livestock industry worldwide, but little is known about the circulation, pathogenicity and genetic diversity of enteric bovine astrovirus (BoAstV) in America. The aim of this work was to describe the prevalence and genetic diversity of enteric BoAstV in dairy cattle in Uruguay. A total of 457 fecal and 43 intestinal contents from dairy calves were collected between July 2015 and May 2017 and tested by RT-PCR, followed by sequencing and phylogenetic analyses of the polymerase and capsid regions. Twenty-six percent (128/500) of the samples were positive. Three different species within the Mamastrovirus genus were identified, including Mamastrovirus 28, Mamastrovirus 33 (3 samples each) and an unclassified Mamastrovirus species (19 samples). The unclassified species was characterized as a novel Mamastrovirus species. BoAstV circulates in Uruguayan dairy cattle with a high genetic diversity. The eventual clinicopathological significance of enteric BoAstV infection in cattle needs further investigation.	
Palabras-Clave: 	BOVINE ASTROVIRUS; DAIRY CATTLE; GENETIC DIVERSITY; MAMASTROVIRUS SPECIES; PLATAFORMA DE SALUD ANIMAL; PREVALENCE.	
Thesagro: 	URUGUAY.	
Categoría del tema: 	L73 Enfermedades de los animales	
URL: 	http://www.ainfo.inia.uy/digital/bitstream/item/16691/1/viruses-12-00032.pdf
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019600/pdf/viruses-12-00032.pdf	
Registro original: 	INIA La Estanzuela (LE)	
Ejemplares: 	LE - (103081-1) - PXI - AP - DD - PP/Viruses/2020 -  -  - DIS