Fecha/hora: 13/06/2024 05:31:20

Biblioteca(s): | INIA Las Brujas.
Fecha corriente: | 02/01/2017
Fecha de la última actualización: | 15/10/2019
Autoria: | CASTILLO, A.; GAIERO, P.; LÓPEZ CARRO, B.; VILARO, F.
Afiliación: | ALICIA MARIA CASTILLO SALLE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; P. GAIERO, Universidad de la República (UdelaR)/ Facultad de Agronomía; B. LÓPEZ CARRO, SECIF-IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); FRANCISCO LUIS VILARO PAREJA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.
Título: | Gametic embryogenic response in wild diploid Solanum species and its implications for genome sequencing projects and breeding.
Año de publicación: | 2016
Fuente/Imprenta: | Plant Tissue Culture and Biotechnology, 2016, v. 26 (2): 159-173.
DOI: | 10.3329/ptcb.v26i2.30566
Idioma: | Inglés

Contenido:

ABSTRACT.

The anther culture response in Solanum commersonii (2n = 2x = 24, 1EBN) and S. chacoense (2n = 2x = 24, 2EBN), two wild potato germplasm resources was studied to obtain haploid plants. Three accessions from each of the two species and 3200 anthers from each genotype were cultured. Authors assessed different culture media; ascorbic acid, L?cysteine and silver nitrate (AgNO3) were included to prevent browning of anther cultures. Addition of AgNO3, was effective to induce embryogenesis. The clones from S. commersonii showed different embryogenic response to androgenesis. However, the three accessions from S. chacoense did not induce any embryo in the same conditions. Ploidy level of the regenerated clones was estimated by flow cytometry and confirmed by chromosome counts. This is the first report of haploid plants obtained from anther culture in S. commersonii, with important implications in sequencing efforts and potato breeding.

Palabras-Clave: | ANTHER CULTURE; DOUBLE HAPLOID; FLOW CYTOMETRY; HAPLOID.
Thesagro: | GERMOPLASMA; PAPA; SOLANUM COMMERSONII.
Categoría del tema: | --

URL:

http://www.ainfo.inia.uy/digital/bitstream/item/13501/1/30566-Article-Text-109802-1-10-20161210.pdf
https://www.banglajol.info/index.php/PTCB/article/view/30566

Registro original: | INIA Las Brujas (LB)
Ejemplares: | LB - (101294-1) - PXI - AP - DD - PP/PTC&Biotech/2016/26(2) -  -  - DIS