Fecha/hora: 13/06/2024 05:38:02

Biblioteca(s): 	INIA La Estanzuela; INIA Tacuarembó.	
Fecha corriente: 	01/12/2020	
Fecha de la última actualización: 	21/05/2021	
Autoria: 	PARODI, P.; CORBELLINI, L.G.; LEOTTI, V.B.; RIVERO, R.; MIRABALLES, C.; RIET-CORREA, F.; VENZAL, J.M.; ARMÚA-FERNÁNDEZ, M.T.	
Afiliación: 	PABLO ANDRÉS PARODI TEXEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LUIS G. CORBELLINI, Laboratório de Epidemiología Veterinária, Facultad de Veterinária, Departamento de Medicina Veterinária Preventiva, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil; VANESSA B. LEOTTI, Departamento de Estatística, Instituto de Matemática e Estatística, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil; RODOLFO RIVERO, Laboratorio Regional Noroeste “Miguel C. Rubino”, División de Laboratorios Veterinarios “Miguel C. Rubino”, Paysandú, Uruguay; MÓNICA CECILIA MIRABALLES FERRER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JOSÉ M. VENZAL, Laboratorio de Vectores y enfermedades transmitidas, Facultad de Veterinaria, CENUR Litoral Norte, Universidad de la República, Salto, Uruguay; MARÍA T. ARMÚA-FERNÁNDEZ, Laboratorio de Vectores y enfermedades transmitidas, Facultad de Veterinaria, CENUR Litoral Norte, Universidad de la República, Salto, Uruguay.	
Título: 	Validation of a multiplex PCR assay to detect Babesia spp. and Anaplasma marginale in cattle in Uruguay in the absence of a gold standard test. (Full Scientific Report)	
Año de publicación: 	2021	
Fuente/Imprenta: 	Journal of Veterinary Diagnostic Investigation, Volume 33, Issue 1, January 2021, Pages 73-79. DOI: https://doi.org/10.1177/1040638720975742	
DOI: 	10.1177/1040638720975742	
Idioma: 	Inglés	
Notas: 	Article history: Article first published online: November 30, 2020; Issue published: January 1, 2021. Acknowledgments: We thank Dra. María Angélica Solari (DILAVE-Montevideo), and the veterinarians and farmers who submitted samples. Corresponding author: Pablo Parodi: pabloparodi7@gmail.com	
Contenido: 	Abstract: Detection of bovine Babesia spp. and Anaplasma marginale is based on the reading of Giemsa-stained blood or organ smears, which can have low sensitivity. Our aim was to improve the detection of bovine Babesia spp. and A. marginale by validating a multiplex PCR (mPCR). We used 466 samples of blood and/or organs of animals with signs and presumptive autopsy findings of babesiosis or anaplasmosis. The primers in our mPCR amplified the rap-1a gene region of Babesia bovis and B. bigemina, and the msp-5 region of A. marginale. We used a Bayesian model with a non-informative priori distribution for the prevalence estimate and informative priori distribution for estimation of sensitivity and specificity. The sensitivity and specificity for smear detection of Babesia spp. were 68.6% and 99.1%, and for A. marginale 85.6% and 98.8%, respectively. Sensitivity and specificity for mPCR detection for Babesia spp. were 94.2% and 97.1%, and for A. marginale 95.2% and 92.7%, respectively. Our mPCR had good accuracy in detecting Babesia spp. and A. marginale, and would be a reliable test for veterinarians to choose the correct treatment for each agent.	
Palabras-Clave: 	ANAPLASMA MARGINALE; BABESIA SPP; CATTLE; ENFERMEDADES DE LOS ANIMALES; MULTIPLEX PCR; PLATAFORMA DE SALUD ANIMAL; SENSITIVITY; SPECIFICITY; TICK-BORNE DISEASES.	
Categoría del tema: 	E16 Enfermedades de los animales	
Registro original: 	INIA La Estanzuela (LE)	
Ejemplares: 	LE - (103275-1) - PXI - AP - DD - PP/JVDI/2021 -  -  - DIS
TBO - (103350-1) - PXI - AP - DD -  -  -  - DIS