Fecha/hora: 12/06/2024 11:17:40

Biblioteca(s): | INIA Las Brujas.
Fecha corriente: | 27/02/2023
Fecha de la última actualización: | 15/03/2023
Autoria: | ROVIRA, P.J.
Afiliación: | PABLO JUAN ROVIRA SANZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.
Título: | Short-term impact of oxytetracycline administration on the fecal microbiome, resistome and virulome of grazing cattle.
Año de publicación: | 2023
Fuente/Imprenta: | Antibiotics, 2023, 12(3):470. OPEN ACCESS. doi: https://doi.org/10.3390/antibiotics12030470
ISSN: | eISSN 2079-6382
DOI: | 10.3390/antibiotics12030470
Idioma: | Inglés

Notas:

Article history: Received 27 December 2022; Revised 14 February 2023; Accepted 22 February 2023; Published 25 February 2023. -- Academic Editor: Carlos M. Franco. -- Funding: This research was funded by the Instituto Nacional de Investigación Agropecuaria (INIA Uruguay), grant number INIA CL 48. The APC was funded by INIA CL 48 and INIA CL 52. -- This article belongs to the Special Issue Antimicrobial Stewardship in Livestock (https://www.mdpi.com/journal/antibiotics/special_issues/Livestock_Antibiotics). -- LICENSE: Licensee MDPI, Basel, Switzerland. This article is an open access article
distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

Contenido: | ABSTRACT.- Antimicrobial resistance (AMR) is an important public health concern around the world. Limited information exists about AMR in grasslands-based systems where antibiotics are seldom used in beef cattle. The present study investigated the impacts of oxytetracycline (OTC) on the microbiome, antibiotic resistance genes (ARGs), and virulence factor genes (VFGs) in grazing steers with no previous exposure to antibiotic treatments. Four steers were injected with a single dose of OTC (TREAT), and four steers were kept as control (CONT). The effects of OTC on fecal microbiome, ARGs, and VFGs were assessed for 14 days using 16S rRNA sequencing and shotgun metagenomics. Alpha and beta microbiome diversities were significantly affected by OTC. Following treatment, less than 8% of bacterial genera had differential abundance between CONT and TREAT samples. Seven ARGs conferring resistance to tetracycline (tet32, tet40, tet44, tetO, tetQ, tetW, and tetW/N/W) increased their abundance in the post-TREAT samples compared to CONT samples. In addition, OTC use was associated with the enrichment of macrolide and lincosamide ARGs (mel and lnuC, respectively). The use of OTC had no significant effect on VFGs. In conclusion, OTC induced short-term alterations of the fecal microbiome and enrichment of ARGs in the feces of grazing beef cattle. Copyright: © 2023 by the author.
Palabras-Clave: | Antibiotic resistance genes; Fecal microbiome; Grazing cattle; Oxytetracycline; Virulence.
Categoría del tema: | L01 Ganadería
URL: | https://www.mdpi.com/2079-6382/12/3/470/pdf
Registro original: | INIA Las Brujas (LB)
Ejemplares: | LB - (103309-1) - PXI - AP - DD - Antibiotics/2023/Rovira -  -  - DIS