Fecha/hora: 03/06/2024 04:35:54

Biblioteca(s): 	INIA Las Brujas; INIA Tacuarembó.	
Fecha corriente: 	19/09/2014	
Fecha de la última actualización: 	15/10/2019	
Autoria: 	DALLA RIZZA, M.; REAL, D.; REYNO, R.; QUESENBERRY, K; BURGUEÑO, J; PORRO, V; ERRICO, E.	
Afiliación: 	MARCO DALLA RIZZA VILARO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; DANIEL REAL FERREIRO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; RAFAEL ALEJANDRO REYNO PODESTA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay.	
Título: 	Genetic diversity and DNA content of three South American and three Eurasiatic Trifolium species	
Año de publicación: 	2007	
Fuente/Imprenta: 	Genetics and Molecular Biology, 2007, v 30, no. 4,  p.1118-1124.	
DOI: 	10.1590/S1415-47572007000600015	
Idioma: 	Inglés	
Notas: 	Article history: Received: March 16, 2007 / Accepted: May 17, 2007..	
Contenido: 	Six species of Trifolium (T. polymorphum Poir., T. riograndense Burkart, T. argentinense Speg., T. medium L., T. pratense L. and T. repens L.) were analyzed using inter-simple sequence repeats (ISSR) markers. Six selected primers generated 186 polymerase chain reaction (PCR) products exploring 112 loci in 34 genotypes analyzed with molecular sizes ranging from 200 to 1300 bp. These primers were able to discriminate among and within species, with the PCR products being on average 41.6% species-specific and 59.9% polymorphic at the within species level. Nuclear DNA content was determined by flow cytometry and revealed variation among species. The 1Cx genome size values were calculated and were found to range from 0.46 pg (T. pratense) to 0.96 pg (T. polymorphum). Genome size values of South American species were higher than those of Eurasiatic origin. The analyses of the molecular data grouped the six species in agreement with their geographical origin and clearly differentiate T. polymorphum from T. argentinense. The Eurasiatic group showed the highest average of species-specific bands (45.3%) and the South American group exhibited the highest amount of total bands (59.7). The highest level of intra-species polymorphisms was detected in T. argentinense (92.9%), followed by T. medium(89.5%).	
Thesagro: 	ADN; PASTURAS; TRIFOLIUM; VARIABILIDAD GENETICA.	
Categoría del tema: 	F30 Genética vegetal y fitomejoramiento	
Registro original: 	INIA Las Brujas (LB)	
Ejemplares: 	LB - (100359-1) - PXI - AP - DD - PP/GENETICS & MOL.BIOL./2007 -  -  - DIS
TBO - (100087-1) - PXI - AP - PP - PP/GENETIC AND MOLECULAR BIOLOGY/2007 -  -  - DIS