Fecha/hora: 04/06/2024 04:51:29

Biblioteca(s): 	INIA La Estanzuela.	
Fecha corriente: 	15/08/2022	
Fecha de la última actualización: 	01/12/2022	
Autoria: 	PASSOS, J.R.S.; GUERREIRO, D.D.; KAMILA S. OTÁVIO; DOS SANTOS-NETO, P.C.; SOUZA-NEVES, M.; CUADRO, F.; NUÑEZ?OLIVERA, R.; CRISPO, M.; VASCONCELOS, F.R.; BEZERRA, M.J.B.; SILVA, R.F.; LIMA, L.F.; FIGUEIREDO, J.R.; BUSTAMANTE-FILHO, I.C.; MENCHACA, A.; MOURA, A.A.	
Afiliación: 	JOSÉ RENATO S. PASSOS, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; DENISE D. GUERREIRO, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; OTÁVIO, K.S., Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; PEDRO C. DOS SANTOS-NETO, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; MARCELA SOUZA-NEVES, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; FEDERICO CUADRO, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; RICHARD NUÑEZ?OLIVERA, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; MARTINA CRIPO, Unidad de Biotecnología en Animales de Laboratorio, Institut Pasteur de Montevideo, Montevideo, Uruguay.; FÁBIO R. VASCONCELOS, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; MARIA JULIA B. BEZERRA, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; RENATO F. SILVA, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), Ceará State University, Fortaleza, Brazil.; LARITZA F. LIMA, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), Ceará State University, Fortaleza, Brazil.; JOSÉ RICARDO FIGUEIREDO, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), Ceará State University, Fortaleza, Brazil.; IVAN C. BUSTAMANTE-FILHO, Laboratório de Biotecnologia, Universidade do Vale do Taquari, Lajeado, Brazil.; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; ARLINDO A. MOURA, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.	
Título: 	How in vitro maturation changes the proteome of ovine cumulus-oocyte complexes?.	
Complemento del título: 	Volume 89, Issue 10, Pages 459 - 470October 2022	
Año de publicación: 	2022	
Fuente/Imprenta: 	Molecular reproduction and development, October 2022, Volume 89, Issue 10, pages 459-470. doi: https://doi.org/10.1002/mrd.23638	
DOI: 	10.1002/mrd.23638	
Idioma: 	Inglés	
Notas: 	Article history: Received: 16 February 2022 | Accepted: 21 July 2022. -- Corresponding author: Moura, A.A.; Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil; email:arlindo.moura@gmail.com -- Funding: The experiments presently described were conducted at the facilities of the Instituto de Reproducción Animal Uruguay (Fundacion IRAUy, Montevideo, Uruguay) and at the Unidad de Biotecnología en Animales de Laboratorio (UBAL) of the Institut Pasteur de Montevideo, Uruguay. Specially, the authors thank Dr. Rosario Durán and Dr. Alejandro Leyva for kindly assisting us in the proteomic experiment. Financial support was provided by Fundacion IRAUy; PRONEX 02/2015 (Programa de Apoio a Núcleos de Excelência Pronex/Funcap/CNPq); The Brazilian Research Council-CNPq (grants # 313160/2017-1 and 438773/2018-7); Brazilian Commission for Higher Education (CAPES); Ceará State Foundation for the Support of Technology and Scientific Development (FUNCAP), Brazil.	
Contenido: 	Abstract: The present study evaluated the effects of in vitro maturation (IVM) on the proteome of cumulus-oocyte complexes (COCs) from ewes. Extracted COC proteins were analyzed by LC-MS/MS. Differences in protein abundances (p < 0.05) and functional enrichments in immature versus in vitro-matured COCs were evaluated using bioinformatics tools. There were 2550 proteins identified in the COCs, with 89 and 87 proteins exclusive to immature and mature COCs, respectively. IVM caused downregulation of 84 and upregulation of 34 proteins. Major upregulated proteins in mature COCs were dopey_N domain-containing protein, structural maintenance of chromosomes protein, ubiquitin-like modifier-activating enzyme 2. Main downregulated proteins in mature COCs were immunoglobulin heavy constant mu, inter-alpha-trypsin inhibitor heavy chain 2, alpha-2-macroglobulin. Proteins exclusive to mature COCs and upregulated after IVM related to immune response, complement cascade, vesicle-mediated transport, cell cycle, and extracellular matrix organization. Proteins of immature COCs and downregulated after IVM were linked to metabolic processes, immune response, and complement cascade. KEGG pathways and miRNA-regulated genes attributed to downregulated and mature COC proteins related to complement and coagulation cascades, metabolism, humoral response, and B cell-mediated immunity. Thus, IVM influenced the ovine COC proteome. This knowledge supports the future development of efficient IVM protocols for Ovis aries. © 2022 Wiley Periodicals LLC.	
Palabras-Clave: 	FOLLICLE; OVARY; OVINE; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL; PLATAFORMA DE SALUD ANIMAL; PROTEINS; REPRODUCTION.	
Categoría del tema: 	--	
Registro original: 	INIA La Estanzuela (LE)	
Ejemplares: 	LE - (103618-1) - PXI - AP - DD - PP/MRD/2022 -  -  - DIS