Fecha/hora: 03/06/2024 05:16:01

Biblioteca(s): | INIA Treinta y Tres.
Fecha corriente: | 14/01/2022
Fecha de la última actualización: | 14/01/2022
Autoria: | MEDEROS, A.; GALARRAGA, D.; VAN DER GRAAF-VAN BLOOIS, L.; BUCZINSKI, S.
Afiliación: | AMERICA ESTHER MEDEROS SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DENISE GALARRAGA, Private Diagnostic Laboratory, Tacuarembo, Uruguay.; LINDA VAN DER GRAAF-VAN BLOOIS, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.; SÉBASTIEN BUCZINSKI, Faculté de Médecine Vétérinaire, Centre d’Expertise Et de Recherche Clinique en Santé Et Bien-Être Animal, Université de Montréal, Département des sciences cliniques, St-Hyacinthe, Canada.
Título: | Performance of bovine genital campylobacteriosis diagnostic tests in bulls from Uruguay: a Bayesian latent class model approach.
Año de publicación: | 2022
Fuente/Imprenta: | Tropical Animal Health and Production, February 2022, volume 54, Article Number 32, pages 1-9. OPEN ACCESS. doi: https://doi.org/10.1007/s11250-021-03039-w
DOI: | 10.1007/s11250-021-03039-w
Idioma: | Inglés
Notas: | Article History: Received: 19 July 2021 / Accepted: 16 December 2021 / Published: 30 December 2021.
Contenido: | The sensitivity (Se) and specificity (Sp) of three diagnostic tests for the detection of Campylobacter fetus venerealis (Cfv) using field samples were estimated using a Bayesian latent class model (BLCM), accounting for the absence of a gold standard. The tests included in this study were direct immunofluorescence antibody test (IFAT), polymerase chain reaction (PCR), and real-time PCR (RT-PCR). Twelve farms from two different populations were selected and bull prepuce samples were collected. The IFAT was performed according to the OIE Manual. The conventional PCR was performed as multiplex, targeting the gene nahE for C. fetus species identification and insertion element ISCfe1 for Cfv identification. The RT-PCR was performed as uniplex: one targeting the gene nahE for C. fetus and the other targeting the insertion ISCfe1 (ISC2) for Cfv. Results from the BLCM showed a median Se of 11.7% (Bayesian credibility interval (BCI): 1.93?29.79%), 53.7% (BCI: 23.1?95.0%), and 36.1% (BCI: 14.5?71.7%) for IFAT, PCR, and RT-PCR respectively. The Sp were 94.5% (BCI: 90.1?97.9%), 97.0% (BCI: 92.9?99.3%), and 98.4% (BCI: 95.3?99.7%) for IFAT, PCR, and RT-PCR respectively. The correlation between PCR and RT-PCR was positive and low in samples from both sampled population (0.63% vs 8.47%). These results suggest that diagnostic sensitivity of the studied tests is lower using field samples than using pure Cfv strains.
Palabras-Clave: | BEEF CATTLE; CAMPYLOBACTER FETUS; Campylobacter fetus subsp. venerealis; FIELD SAMPLES; LATENT CLASS MODEL.
Categoría del tema: | L73 Enfermedades de los animales
URL: | http://www.ainfo.inia.uy/digital/bitstream/item/16181/1/TAHP-2002-Mederos.pdf
Registro original: | INIA Treinta y Tres (TT)
Ejemplares: | TT - (103464-1) - PXI - AP - DD - Trop.Anim.Health.Prod./2022-Mederos -  -  - DIS