Fecha/hora: 06/06/2024 12:58:46

Biblioteca(s): | INIA Las Brujas.
Fecha corriente: | 27/06/2023
Fecha de la última actualización: | 28/06/2023
Autoria: | DE TRAVENSOLO, R.F.; FERREIRA, V.G.; FEDERICI, M.; DE LEMOS, E.G.M.; CARRILHO, E.
Afiliación: | REGIANE FÁTIMA DE TRAVENSOLO, Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil; VINÍCIUS GUIMARÃES FERREIRA, Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil; MARIA TERESA FEDERICI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ELIANA GERTRUDES MACEDO DE LEMOS, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Via de Acesso Prof. Paulo Donato Castellane, Jaboticabal, SP, Brazil; EMANUEL CARRILHO, Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil.
Título: | Microarrays application in life sciences: The beginning of the revolution.
Año de publicación: | 2022
Fuente/Imprenta: | In: L. T. Kubota et al. (eds.). 2022. Tools and Trends in Bioanalytical Chemistry. Springer. Pages 483-496. https://doi.org/10.1007/978-3-030-82381-8_25
ISBN: | 978-303082380-1; eISBN: 978-303082381-8
DOI: | 10.1007/978-3-030-82381-8_25
Idioma: | Inglés
Notas: | Correspondence: Carrilho, E.; Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil; email:emanuel@iqsc.usp.br --
Contenido: | Previous to Next Generation Sequencing (NGS), a different technology allowed science to drive through the roads of genetic analysis, the microarray technology. Shortly, microarrays consist of a grid of spots, with each spot containing single-stranded DNA sequences attached to a solid surface. For microarray analysis, the mRNA must be extracted from the sample and converted to a labeled cDNA strand, and later added to the microarray device. The DNA sequences on the microchip are fragments of genes of interest, and once the sample is added to the microarray, the cDNA strands from the sample hybridize with the strands fixed on the device if they are matching. In that way, a single device allows the analyst to check for the presence of several genes at once. In this chapter, the reader will learn precisely how microarray analysis works, concepts to produce in-lab microarrays, and the main research areas applying the microarray technology. © The Author(s), under exclusive license to Springer Nature Switzerland AG 2022. All rights reseverd.
Palabras-Clave: | DNA analysis; Microarray applications; Microarrays.
Categoría del tema: | F30 Genética vegetal y fitomejoramiento
Registro original: | INIA Las Brujas (LB)
Ejemplares: | LB - (103556-1) - PXI - PL - DD - Springer/2022 -  -  - DIS