Fecha/hora: 10/06/2024 15:23:08

Biblioteca(s): | INIA Las Brujas.
Fecha corriente: | 29/01/2024
Fecha de la última actualización: | 29/01/2024
Autoria: | TORRES, P.; ALTIER, N.; BEYHAUT, E.; FRESIA, P.; GARAYCOCHEA, S.; ABREO, E.
Afiliación: | PABLO ANDRES TORRES ASTETE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; NORA ADRIANA ALTIER MANZINI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ELENA BEYHAUT GUTIERREZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PABLO FRESIA, Unidad Mixta UMPI, Institut Pasteur Montevideo + INIA, Montevideo, Uruguay; SILVIA RAQUEL GARAYCOCHEA SOLSONA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; EDUARDO RAUL ABREO GIMENEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.
Título: | Phenotypic, genomic and in planta characterization of Bacillus sensu lato for their phosphorus biofertilization and plant growth promotion features in soybean.
Año de publicación: | 2024
Fuente/Imprenta: | Microbiological Research, 2024, Volume 280, e127566. https://doi.org/10.1016/j.micres.2023.127566
ISSN: | 0944-5013
DOI: | 10.1016/j.micres.2023.127566
Idioma: | Inglés
Notas: | Article history: Received 12 November 2023; Received in revised form 30 November 2023; Accepted 2 December 2023; Available online 6 December 2023. -- Correspondence: Abreo, E.; Estación Experimental Wilson Ferreira Aldunate, Ruta 48 km 10, Canelones, Uruguay; email:eabreo@inia.org.uy  -- Funding: This research was funded by Agencia Nacional de Investigación e Innovación ( ANII, Proyecto ALI_1_2014_1_5046 ), Instituto Nacional de Investigación Agropecuaria (INIA Uruguay), Institut Pasteur de Montevideo , Calister, Lage and Lafoner companies. --
Contenido: | ABSTRACT.- Bacillus sensu lato were screened for their capacity to mineralize organic phosphorus (P) and promote plant growth, improving nitrogen (N) and P nutrition of soybean. Isolates were identified through Type Strain Genome Server (TYGS) and Average Nucleotide Identity (ANI). ILBB95, ILBB510 and ILBB592 were identified as Priestia megaterium, ILBB139 as Bacillus wiedmannii, ILBB44 as a member of a sister clade of B. pumilus, ILBB15 as Peribacillus butanolivorans and ILBB64 as Lysinibacillus sp. These strains were evaluated for their capacity to mineralize sodium phytate as organic P and solubilize inorganic P in liquid medium. These assays ranked ILBB15 and ILBB64 with the highest orthophosphate production from phytate. Rhizocompetence and plant growth promotion traits were evaluated in vitro and in silico. Finally, plant bioassays were conducted to assess the effect of the co-inoculation with rhizobial inoculants on nodulation, N and P nutrition. These bioassays showed that B. pumilus, ILBB44 and P. megaterium ILBB95 increased P-uptake in plants on the poor substrate of sand:vermiculite and also on a more fertile mix. Priestia megaterium ILBB592 increased nodulation and N content in plants on the sand:vermiculite:peat mixture. Peribacillus butanolivorans ILBB15 reduced plant growth and nutrition on both substrates. Genomes of ILBB95 and ILBB592 were characterized by genes related with plant growth and biofertilization, whereas ILBB15 was differentiated by genes related to bioremediation. Priestia megaterium ILBB592 is considered as nodule-enhancing rhizobacteria and together with ILBB95, can be envisaged as prospective PGPR with the capacity to exert positive effects on N and P nutrition of soybean plants. © 2023 Elsevier GmbH
Palabras-Clave: | Auxin; Biofilm; Nitrogen; Nodulation; PLATAFORMA DE BIOINSUMOS - INIA; Priestia; Rhizobia.
Categoría del tema: | --
Registro original: | INIA Las Brujas (LB)
Ejemplares: | LB - (103790-1) - PXI - AP - DD - Microbiological Research/2024 -  -  - DIS