Fecha/hora: 05/06/2024 11:15:52

Biblioteca(s): | INIA Las Brujas.
Fecha corriente: | 21/02/2014
Fecha de la última actualización: | 30/10/2019
Autoria: | DALLA RIZZA, M.; REAL, D.; QUESENBERRY, K.H.; ALBERTINI, E.
Afiliación: | MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DANIEL REAL FERREIRO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; K.H. QUESENBERRY, Dep. of Agronomy, Univ. of Florida, USA; E. ALBERTINI, Dipartimento di Biologia Vegetale e Biotecnologie Agroambientali, University of Perugia, Italy.
Título: | Plant reproductive system determination under field conditions based on condominant markers.
Año de publicación: | 2004
Fuente/Imprenta: | Journal of Genetics and Breeding, 2004, Volume 58, Issue 1, pp 47-54.
Descripción Física: | 2-s2.0-33748791330
ISSN: | 0394-9257
Idioma: | Inglés
Notas: | Article history: Received February 9, 2004.

Contenido:

ABSTRACT.
Two co-dominant PCR-based molecular tools, sequence characterized amplified regions (SCAR) markers and restriction enzymes mediated markers, sequence characterized amplified regions (CAPS) markers, have been utilized to study the mating system in Lotononis bainesii Baker. Randomly amplified polymorphic DNA (RAPD) phenotypes - bands on a gel - were selected to transform into specific SCAR genotypes - allelic constitution at a set of loci. Moreover, some monomorphic SCAR genotypes, were made polymorphic through the use of restriction enzymes, in order to analyze allelic contribution on progenies. Based on stringent annealing temperatures that generate only specific DNA fragments and only in those individuals possessing the appropriate allele, it was possible to recognize alleles from different donors. This approach overcomes the limitation of different dominant markers and has the advantage of analyzing a single genetic locus PCR-based, avoiding the necessity of complex and time consuming controlled crosses. Thus, more suitable for reproductive system studies based on the use of molecular characterized plants that acts as "tester genotypes". In this article we discuss the advantages of using two independent and conclusive molecular approaches based on SCAR and CAPS markers to determine plant breeding systems. We also show that allogamy is the mode of reproduction of Lotononis bainesii.

Palabras-Clave: | Mating system; SCAR/CAPS genotypes.
Thesagro: | LOTONONIS BAINESII.
Categoría del tema: | F03 Producción y tratamiento de semillas
Registro original: | INIA Las Brujas (LB)
Ejemplares: | LB - (12504-1) - PXI - AP - DD - PP/JR.Genetics&Breeding/2004 -  -  - DIS