Fecha/hora: 09/06/2024 22:12:11

Biblioteca(s): 	INIA Las Brujas.	
Fecha corriente: 	22/01/2020	
Fecha de la última actualización: 	22/01/2020	
Autoria: 	PAZ, L.N.; HAMOND, C.; DIAS, C.S.; CURVELO, V.P.; MEDEIROS, M.A.; ORIÁ, A.P.; PINNA, M.H.	
Afiliación: 	LUCAS NOGUEIRA PAZ, School of Veterinary Medicine and Zootechny, Federal University of Bahia, Salvador, BA, Brazil; CAMILA HAMOND, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLA SILVA DIAS, School of Veterinary Medicine and Zootechny, Federal University of Bahia, Salvador, BA, Brazil; VITOR P. CURVELO, Getúlio Vargas Zoobotanic Park, Salvador, BA, Brazil; MARCO ALBERTO MEDEIROS, Laboratory of Recombinant Technology, Bio-Manguinhos, Brazilian Ministry of Health, FIOCRUZ, Brazil; ARIANNE PONTES ORIÁ, School of Veterinary Medicine and Zootechny, Federal University of Bahia, Salvador, BA, Brazil; MELISSA HANZEN PINNA, School of Veterinary Medicine and Zootechny, Federal University of Bahia, Salvador, BA, Brazil.	
Título: 	Detection of Leptospira spp. in Captive Broad-Snouted Caiman (Caiman latirostris). [Original Contribution]	
Año de publicación: 	2019	
Fuente/Imprenta: 	EcoHealth, 1 December 2019, Volume 16, Issue 4, Pages 694-700. Doi: 10.1007/s10393-019-01452-0	
ISSN: 	1612-9202	
DOI: 	10.1007/s10393-019-01452-0	
Idioma: 	Inglés	
Notas: 	Article history: Received 12 November 2018 / Accepted 30 September 2019 / Published Online 07 November 2019.
The online version of this article contains supplementary material, which is available to authorized users.	
Contenido: 	ABSTRACT.
Leptospira sp. is an important waterborne zoonotic bacterium, known to cause infection in animals and humans worldwide. The role of reptiles in the transmission of this microorganism is poorly understood and historically neglected. This study aimed to investigate the presence of anti-Leptospira spp. antibodies and leptospiral DNA in captive Caiman latirostris (broad-snouted caiman). Of the 23 reptiles studied by microscopic agglutination test (MAT), 22/23 (95.65%) were considered reactive (titers &#8805; 100) and 1/23 (4.35%) non-reactive (titer < 100). The serogroup with highest occurrence was Grippotyphosa (68.18%, n = 15/22) followed by serogroup Djasiman (18.18%, n = 4/22). Specific amplification of Leptospira spp. gene lipL32 was observed in six (26.09%, n = 6/23) blood samples. Five of six samples, previously detected as pathogenic leptospira by PCR, were amplified and sequenced. All the samples corresponded to the pathogenic species Leptospira interrogans (presented 100% of identity) using the PCR targeting to secY gene. We demonstrated high detection of DNA of L. interrogans in crocodilians, and the authors suggest that further research is needed to elucidate the impact of Leptospira spp. infection in health broad-snouted caimans as well as the pathophysiology of leptospirosis in crocodilians. 
© 2019, EcoHealth Alliance.	
Palabras-Clave: 	Crocodilian; Diagnosis; PLATAFORMA SALUD ANIMAL.	
Thesagro: 	LEPTOSPIROSIS; REPTILES; ZOONOSIS.	
Categoría del tema: 	A50 Investigación agraria	
Registro original: 	INIA Las Brujas (LB)	
Ejemplares: 	LB - (102106-1) - PXI - AP - DD - PP/EcoHealth/2019 -  -  - DIS