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Biblioteca(s): 	INIA Treinta y Tres.	
Fecha corriente: 	14/01/2021	
Fecha de la última actualización: 	15/01/2021	
Autoria: 	NZOYIKORERA, N.; DIAWARA, I.; FRESIA, P.; MAALOUM, F.; KATFY, K.; NAYME, K.; MAALOUM, M.; COMICK, J.; CHAGUZA, C.; TIMINOUNI, M.; BELABESS, H.; ZEROUALI, K.; ELMDAGHRI, N.	
Afiliación: 	NÉHÉMIE NZOYIKORERA, Department of Microbiology, Faculty of Medicine and Pharmacy of Casablanca, Hassan II University of Casablanca, Casablanca, Morocco. Bacteriology-Virology and Hospital Hygiene Laboratory, Ibn Rochd University Hospital Centre, Casablanca, Morocco; IDRISSA DIAWARA, Faculty of Sciences and Health Techniques, Mohammed VI University of Health Sciences (UM6SS) of Casablanca, Casablanca, Morocco.; PABLO FRESIA, Unidad Mixta UMPI, Institut Pasteur Montevideo + INIA, Montevideo, Uruguay; FAKNREDDINE MAALOUM, Department of Microbiology, Faculty of Medicine and Pharmacy of Casablanca, Hassan II University of Casablanca, Casablanca, Morocco. Bacteriology-Virology and Hospital Hygiene Laboratory, Ibn Rochd University Hospital Centre, Casablanca, Morocco.; KHALID KATFI, Department of Microbiology, Faculty of Medicine and Pharmacy of Casablanca, Hassan II University of Casablanca, Casablanca, Morocco.; KAOTAR NAYME, Molecular Bacteriology Laboratory, Institut Pasteur du Maroc, Casablanca, Morocco. 9Laboratory of Biology and Health, Faculty of Sciences Ben M'Sik, Hassan II University of Casablanca, Casablanca, Morocco.; MOSSAAB MAALOUM, Molecular Bacteriology Laboratory, Institut Pasteur du Maroc, Casablanca, Morocco. 9Laboratory of Biology and Health, Faculty of Sciences Ben M'Sik, Hassan II University of Casablanca, Casablanca, Morocco.; JENNIFER COMICK, Malawi Liverpool Wellcome Trust Clinical Research Programme, Blantyre, Malawi. Institute of Infection and Global Health, University of Liverpool, Liverpool, UK.; CHRISPIN CHAGUZA, Wellcome Trust Sanger Institute, Cambridge, UK.; MOHAMMED TIMINOUNI, Molecular Bacteriology Laboratory, Institut Pasteur du Maroc, Casablanca, Morocco.; HOURIA BELABESS, Department of Microbiology, Faculty of Medicine and Pharmacy of Casablanca, Hassan II University of Casablanca, Casablanca, Morocco.Bacteriology-Virology and Hospital Hygiene Laboratory, Ibn Rochd University Hospital Centre, Casablanca, Morocco.; KHALID ZEROUALI, Department of Microbiology, Faculty of Medicine and Pharmacy of Casablanca, Hassan II University of Casablanca, Casablanca, Morocco..Bacteriology-Virology and Hospital Hygiene Laboratory, Ibn Rochd University Hospital Centre, Casablanca, Morocco.; NAIMA ELMDAGHRI, Department of Microbiology, Faculty of Medicine and Pharmacy of Casablanca, Hassan II University of Casablanca, Casablanca, Morocco. Bacteriology-Virology and Hospital Hygiene Laboratory, Ibn Rochd University Hospital Centre, Casablanca, Morocco.	
Título: 	Whole genomic comparative analysis of Streptococcus pneumoniae serotype 1 isolates causing invasive and non-invasive infections among children under 5 years in Casablanca, Morocco. [Open Access].	
Año de publicación: 	2021	
Fuente/Imprenta: 	BMC Genomics. 2021 Jan 7;22(1):39. OPEN ACCESS. Doi: 10.1186/s12864-020-07316-0.	
DOI: 	10.1186/s12864-020-07316-0	
Idioma: 	Inglés	
Notas: 	Article history: Received: 19 July 2020 / Accepted: 9 December 2020 / Published online 07 January 2021.
Correspondence: nzoyikorera@yahoo.fr	
Contenido: 	Abstract
Background: Streptococcus pneumoniae serotype 1 remains a leading cause of invasive pneumococcal diseases, even in countries with PCV-10/PCV-13 vaccine implementation. The main objective of this study, which is part of the Pneumococcal African Genome project (PAGe), was to determine the phylogenetic relationships of serotype 1 isolates recovered from children patients in Casablanca (Morocco), compared to these from other African countries; and to investigate the contribution of accessory genes and recombination events to the genetic diversity of this serotype.

Results: The genome average size of the six-pneumococcus serotype 1 from Casablanca was 2,227,119 bp, and the average content of coding sequences was 2113, ranging from 2041 to 2161. Pangenome analysis of the 80 genomes used in this study revealed 1685 core genes and 1805 accessory genes. The phylogenetic tree based on core genes and the hierarchical bayesian clustering analysis revealed five sublineages with a phylogeographic structure by country. The Moroccan strains cluster in two different lineages, the five invasive strains clusters altogether in a divergent clade distantly related to the non-invasive strain, that cluster with all the serotype 1 genomes from Africa.

Conclusions: The whole genome sequencing provides increased resolution analysis of the highly virulent serotype 1 in Casablanca, Morocco. Our results are concordant with previous works, showing that the phylogeography of S. pneumoniae serotype 1 is structured by country, and despite the small size (six isolates) of the Moroccan sample, our analysis shows the genetic cohesion of the Moroccan invasive isolates.	
Palabras-Clave: 	COMPARATIVE GENOMIC ANALYSIS; PNEUMOCCAL AFRICAN GENOME PROJECT (PAGe); SEROTYPE 1; STREPTOCOCCUS PNEUMONIAE; WHOLE GENOME SEQUENCING.	
Categoría del tema: 	--	
URL: 	http://www.ainfo.inia.uy/digital/bitstream/item/14929/1/BMC-2021.pdf	
Registro original: 	INIA Treinta y Tres (TT)	
Ejemplares: 	TT - (103223-1) - PXI - AP - DD - PP/BMC GENOMICS/2021 -  -  - DIS