Fecha/hora: 08/06/2024 02:57:53

Biblioteca(s): | INIA Las Brujas.
Fecha corriente: | 21/02/2014
Fecha de la última actualización: | 05/12/2018
Autoria: | BAYEMI, P. H.; NSONGKA, V. M.; PERERA, B. M. A. O.; CAVESTANY, D.; WEBB, E. C.
Afiliación: | P. H. BAYEMI, Institute of Agricultural Research for Development, Cameroon; V. M. NSONGKA, Institute of Agricultural Research for Development, Cameroon; B. M. A. O. PERERA, University of Peradeniya, Sri Lanka; DANIEL CAVESTANY BOCKING, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; E. C. WEBB, Department of Animal and Wildlife Sciences, University of Pretoria, South Africa.
Título: | Validation of a human progesterone enzyme immunoassay (EIA) kit for use on serum of cattle in Cameroon.
Año de publicación: | 2007
Fuente/Imprenta: | Tropical Animal Health and Production, June 2007, vol. 39, Issue 5, pages 335-338.
ISSN: | 0049-4747 // Online ISSN 1573-7438
DOI: | 10.1007/s11250-007-9020-6
Idioma: | Inglés
Notas: | Article history: Accepted: 8 March 2007 // Published online: 5 June 2007.

Contenido:

ABSTRACT.
A study aimed at validating a human progesterone enzyme immunoassay kit was carried out on cattle at Bambui, Cameroon. Progesterone ELISA Kits (EH-511) were obtained from Clinpro International. Forty-one cows were selected, of which 19 were pregnant and 22 within 14 days post partum. Blood samples were analysed and progesterone levels were deduced from a curve obtained from standard absorbance values (A 450). Results show that 95.5% of postpartum cows had progesterone levels below 1 ng/ml, with the highest level being 0.75 ng/ml. The mean level was 0.5 ± 0.26 ng/ml. The cows in the 'pregnant group' had progesterone levels ranging from 3.5 to 17.5 ng/ml. This kit can be used for measuring progesterone levels in cattle. Levels of 1 ng/ml for two consecutive samples or one sample at or above 3 ng/ml are an indication of the presence of corpus luteum, while cows below 1 ng/ml will be in anoestrus. © 2007 Springer Science + Business Media B.V.

Palabras-Clave: | BEEF CATTLE; CAMEROON; CATTLE; ELISA; IMMUNOASSAY; PROGESTERONE; VALIDATION.
Categoría del tema: | --
Registro original: | INIA Las Brujas (LB)
Ejemplares: | LB - (2932-1) - PXI - AP - DD - PP/Tropical Animal Health & Prod./2007 -  -  - DIS