Fecha/hora: 03/06/2024 08:41:44

Biblioteca(s): 	INIA Las Brujas.	
Fecha corriente: 	07/06/2022	
Fecha de la última actualización: 	30/11/2022	
Autoria: 	MACHADO, M.; CASTRO, M.B.; WILSON, T. M.; GONÇALVES , A. A. B.; PORTIANSKY, E.; RIET-CORREA, F.; BARROS, S. S.	
Afiliación: 	MIZAEL MACHADO DA COSTA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MÁRCIO B. CASTRO, University of Brasilia, Brasília, Brazil; TAIS M. WILSON, University of Brasilia, Brasília, Brazil; ALEXANDRA A. B. GONÇALVES, University of Brasilia, Brasília, Brazil; ENRIQUE L. PORTIANSKY, National University of La Plata, La Plata, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Federal University of Bahia, Salvador, Brazil; SEVERO S. BARROS, Federal University of Santa Maria, Rio Grande do Sul, Brazil.	
Título: 	Poisoning by Nierembergia veitchii: Effects on vascular smooth muscle cells in the pathogenesis of enzootic calcinosis.	
Año de publicación: 	2022	
Fuente/Imprenta: 	Veterinary Pathology, 2022, Volume 59, Issue 5, pages 814-823. doi: https://doi.org/10.1177/03009858221098430	
ISSN: 	0300-9858	
DOI: 	10.1177/03009858221098430	
Idioma: 	Inglés	
Notas: 	Article history: Article first published online May 19, 2022; Published online September 2022. -- Corresponding Author: Franklin Riet-Correa, Postgraduate Program in Animal Science in the Tropics, School of Veterinary Medicine and Animal Science, Federal University of Bahia, Salvador, Bahia 40170-110, Brazil. Email: franklinrietcorrea@gmail.com  --  This work was funded by National Institute of Agricultural Research (INIA) of Uruguay (Project CL 44), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil, (Finance Code 001) and National University of La Plata (Project V270). A postgraduate scholarship was provided to Mizael Machado by INIA, Uruguay.	
Contenido: 	ABSTRACT - Vascular mineralization is a hallmark of enzootic calcinosis. Histopathological, ultrastructural, and immunohistochemical investigations were performed on the external carotid arteries of seven sheep naturally poisoned by Nierembergia veitchii. Histologically, moderate to marked hyperplasia of the tunica intima was observed without mineralization. The tunica media exhibited mild to severe mineralization and osteochondroid metaplasia. Sheep with enzootic calcinosis showed arterial overexpression of osteopontin and tissue-nonspecific alkaline phosphatase and immunolabeling for osteonectin and osteocalcin in both intima and media layers of the tested arteries. The main ultrastructural finding in the tunica media was a marked phenotypic change of vascular smooth muscle cells from a contractile phenotype (VSMC-C) into a synthetic phenotype (VSMC-S). In the tunica media, VSMC-S produced matrix and extracellular vesicles, forming mineralizable granules associated with arterial mineralization. VSMC-S were also present in the tunica intima, but matrix and extracellular vesicles and mineralization were not observed. The absence of matrix and extracellular vesicles in the intimal hyperplasia, even in the presence of noncollagenous bone proteins, tissue-nonspecific alkaline phosphatase, and vitamin D receptors, reinforces the hypothesis that the presence of matrix and extracellular vesicles are crucial for the development of vascular mineralization in enzootic calcinosis. It is proposed that the two different VSMC-S phenotypes in calcinosis are due to the expression of at least two genetically different types of these cells induced by the action of 1,25(OH)2D3. © The Author(s) 2022.	
Palabras-Clave: 	Arterial mineralization; Arteries; Enzootic calcinosis; Exosomes; Pathogenesis; PLATAFORMA DE SALUD ANIMAL; Toxicity; Vascular smooth muscle cells.	
Categoría del tema: 	L50 Fisiología y bioquímica animal	
Registro original: 	INIA Las Brujas (LB)	
Ejemplares: 	LB - (103089-1) - PXI - PC - DD - Veterinary Pathology/2022 -  -  - DIS