Fecha/hora: 23/05/2024 03:28:34

Biblioteca(s): 	INIA La Estanzuela.	
Fecha corriente: 	18/12/2020	
Fecha de la última actualización: 	18/12/2020	
Autoria: 	DORSCH, M.; REGIDOR-CERRILLO, J.; ORTEGA-MORA, L.M.; SCIOLI, M.V.; MORRELL, E.L.; CANTÓN, G.J.; MOORE, D.P.; HECKER, Y.P.	
Afiliación: 	MATÍAS ANDRÉS DORSCH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay;Faculty of Agrarian Sciences, National University of Mar del Plata (UNMdP), Mar del Plata, Argentina; JAVIER REGIDOR-CERRILLO, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Madrid, Spain.; LUIS M. ORTEGA-MORA, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Madrid, Spain.; MARÍA V. SCIOLI, María V. Scioli National Institute of Agrarian Technology (INTA), Buenos Aires, Argentina.; ELEONORA L. MORRELL, National Institute of Agrarian Technology (INTA), Buenos Aires, Argentina.; GERMÁN J. CANTÓN, National Institute of Agrarian Technology (INTA), Buenos Aires, Argentina.; DADÍN P. MOORE, Faculty of Agrarian Sciences, National University of Mar del Plata (UNMdP), Mar del Plata, Argentina.; YANINA P. HECKER, National Institute of Agrarian Technology (INTA), Buenos Aires, Argentina.	
Título: 	Microsatellite genotyping reveals extensive genetic diversity in bovine Neospora caninum from the humid Pampa region in Argentina.	
Año de publicación: 	2020	
Fuente/Imprenta: 	Parasitology Research, December 2020, Volume 119, Issue 12, Pages 4049-4059. Doi:  https://doi-org/10.1007/s00436-020-06922-x	
DOI: 	10.1007/s00436-020-06922-x	
Idioma: 	Inglés	
Notas: 	Article history:Received: 12 August 2020 /Accepted: 5 October 2020	
Contenido: 	Abstract: Neospora caninum is an apicomplexan protozoan and a major cause of abortion in cattle worldwide. In the Argentinian Humid Pampa, bovine neosporosis causes severe economic losses. Despite this, information on the genetic structure of N. caninum in this region is limited. Therefore, this study aimed to genetically characterize N. caninum isolates associated with bovine abortion in the Humid Pampa region. For this purpose, spontaneous bovine fetal tissues submitted for diagnosis to the Veterinary Diagnostic Service at INTA Balcarce during 2008?2019 were assessed by PCR, indirect fluorescent antibody test (IFAT), and histologic analysis. PCR-positive samples were tested by multilocus microsatellite genotyping (MLGs) using 9 microsatellite markers. Thirty-one different genotypes were identified from 32 samples with at least seven markers. Argentinian MLGs were grouped into two clonal clusters when analyzed using eBURST network and principal coordinate analysis. No segregation based on the year of collection, animal biotype, or geographic origin was observed. In addition, the presence of linkage disequilibrium supported the clonal propagation of Argentinian MLGs. One Argentinian subpopulation was associated with isolates from Spain, Uruguay, Brazil, and Mexico, and the other one was linked to isolates from Scotland, Spain, and Germany. These findings reveal the presence of two clonal subpopulations of N. caninum in the Humid Pampa.	
Palabras-Clave: 	ABORTION; BOVINE; MICROSATELLITE MARKERS; NEOSPORA CANINUM; PLATAFORMA SALUD ANIMAL.	
Thesagro: 	ARGENTINA.	
Categoría del tema: 	--	
Registro original: 	INIA La Estanzuela (LE)	
Ejemplares: 	LE - (103260-1) - PXI - AP - DD - PP/PR/ 2020 -  -  - DIS