Fecha/hora: 07/06/2024 15:08:00

Biblioteca(s): | INIA Las Brujas.
Fecha corriente: | 25/09/2016
Fecha de la última actualización: | 09/10/2019
Autoria: | ARRUABARRENA, A.; BENITEZ-GALEANO, M.J.; GIAMBIASI, M.; BERTALMIO, A.; COLINA, R.; HERNÁNDEZ-RODRÍGUEZ, L.
Afiliación: | ANA ARRUABARRENA PASCOVICH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA JOSÉ BENÍTEZ-GALEANO, Laboratorio de Virología Molecular, Centro Universitario Regional Noroeste (CENUR Noroeste), Universidad de la República; MARIO ALEJANDRO GIAMBIASI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA MARIA BERTALMIO CASARIEGO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RODNEY COLINA, Laboratorio de Virología Molecular, Centro Universitario Regional Noroeste (CENUR Noroeste), Universidad de la República; LESTER HERNÁNDEZ-RODRÍGUEZ, Instituto de Investigaciones en Fruticultura Tropical, La Habana, Cuba.
Título: | Application of a simple and affordable protocol for isolating plant total nucleic acids for RNA and DNA virus detection.
Año de publicación: | 2016
Fuente/Imprenta: | Journal of Virological Methods, 2016, v.237, p. 14-17.
DOI: | 10.1016/j.jviromet.2016.08.011
Idioma: | Inglés
Notas: | Article history: Received 30 May 2016 / Received in revised form 26 July 2016 / Accepted 14 August 2016 / Available online 16 August 2016.

Contenido:

ABSTRACT. 

Standard molecular methods for plant virus diagnosis require the purification of RNA or DNA extracts from a large number of samples, with sufficient concentration and quality for their use in PCR, RT-PCR, or qPCR analysis. Most methods are laborious and use either hazardous and/or costly chemicals.Apreviously published protocol for RNA isolation from several plant species yields high amounts of good quality RNADNA mixture in a simple, safe and inexpensive manner. In the present work, this method was tested to obtain RNA-DNA extracts from leaves of tomato, potato and three species of citrus, and was compared with two commercial kits. The results demonstrated that this protocol offers at least comparable nucleic
acid quality, quantity and purity to those provided by commercial phenol-based or spin column systems and that are suitable to be used in PCR, RT-PCR and qPCR for virus and viroid detection. Because of its easy implementation and the use of safe and inexpensive reagents, it can be easily implemented to work in plant virus and viroid detection in different plant species.

© 2016 Elsevier B.V. All rights reserved

Palabras-Clave: | DNA; PCR; PLANT VIROID; PLANT VIRUS; PURIFICATION; RNA; RT-PCR.
Thesagro: | CITRUS.
Categoría del tema: | --
Registro original: | INIA Las Brujas (LB)
Ejemplares: | LB - (101139-1) - PXI - AP - DD - PP/JR.VIROLOGICAL METHODS/2016 -  -  - DIS