Fecha/hora: 08/06/2024 05:42:47

Biblioteca(s): | INIA Treinta y Tres.
Fecha corriente: | 12/09/2014
Fecha de la última actualización: | 08/10/2019
Autoria: | CARLOMAGNO, M.; MATHO, C.; CANTOU, G.; SANBORN, J.R.; LAST, J.A.; HAMMOCK, B.D.; ROEL, A.; GONZÁLEZ, D.; GONZÁLEZ-SAPIENZA, G.
Afiliación: | MARIA GUILLERMINA CANTOU MAYOL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ALVARO ROEL DELLAZOPPA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay.
Título: | A clomazone immunoassay to study the environmental fate of the herbicide in rice (Oryza sativa) agriculture.
Año de publicación: | 2010
Fuente/Imprenta: | Journal of Agricultural and Food Chemistry, 2010, v. 58, no.7,  p. 4367-4371.
ISSN: | 0021-8561
DOI: | 10.1021/jf9043259
Idioma: | Inglés
Notas: | Article history:  received for review December 7, 2009. Revised manuscript received March 8, 2010. Accepted March 10, 2010. Published on Web 03/19/2010.

Contenido:

Abstract:
The environmental impact of rice agriculture is poorly studied in developing countries, mainly due to limitations of the analytical capacity. Here, we report the development of a clomazone enzymelinked immunosorbent assay as a fast and cost-effective tool to monitor the dissipation of this herbicide along the harvest. Antibodies were prepared using different strategies of hapten conjugation, and the best hapten/antibody pair was selected. It proved to be a reliable tool to measure the herbicide in the 2.0-20 ng/mL range in field samples, with excellent correlation with highperformance liquid chromatography results. The assay was used to study the dissipation of the herbicide in the floodwater of experimental rice paddies in Uruguay. Large differences in the residual amounts of herbicide were observed depending on the flooding practices. Because of its robustness and simplicity, the assay may be useful to delineate and monitor management practices that can
contribute to minimizing the release of the herbicide in the environment.

Palabras-Clave: | ELISA; HPLC; RICE.
Thesagro: | ARROZ; CLOMAZONE; CONTROL DE MALEZAS; ESCARDA; HERBICIDAS; ORYZA SATIVA.
Categoría del tema: | H60 Malezas y escardas
Registro original: | INIA Treinta y Tres (TT)
Ejemplares: | TT - (100033-1) - PXI - AP - DD - PP/Roel/Arb/2010/1 -  -  - DIS