Fecha/hora: 11/06/2024 08:03:12

Biblioteca(s): | INIA Treinta y Tres.
Fecha corriente: | 09/09/2014
Fecha de la última actualización: | 25/09/2018
Autoria: | ROSAS, J.; BONNECARRERE, M.; PÉREZ DE VIDA, F.
Afiliación: | JUAN EDUARDO ROSAS CAISSIOLS, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MARIA VICTORIA BONNECARRERE MARTINEZ, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; FERNANDO BLAS PEREZ DE VIDA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.
Título: | One-step, codominant detection of imidazolinone resistance mutations in weedy rice (Oryza sativa L.).
Año de publicación: | 2014
Fuente/Imprenta: | Electronic Journal of Biotechnology, 2014, v. 17, no. 2, p.95-101
ISSN: | 0717-3458
DOI: | 10.1016/j.ejbt.2014.02.003
Idioma: | Inglés
Notas: | Article history: received 25 June 2013; accepted 15 January 2014; available online 16 February 2014.

Contenido:

Abstract
Weedy rice (Oryza sativa L.) is a noxious form of cultivated rice (O. sativa L.) associated with intensive rice production and dry seeding. A cost-efficient strategy to control this weed is the Clearfield rice production system, which combines imidazolinone herbicides with mutant imidazolinone-resistant rice varieties. However, imidazolinone resistance mutations can be introgressed in weedy rice populations by natural outcrossing, reducing the life span of the Clearfield technology. Timely and accurate detection of imidazolinone resistance mutations in weedy rice may contribute to avoiding the multiplication and dispersion of resistant weeds and to protect the Clearfield system. Thus, highly sensitive and specific methods with high throughput and low cost are needed. KBioscience?s Allele Specific PCR (KASP) is a codominant, competitive allele-specific PCR-based genotyping method. KASP enables both alleles to be detected in a single reaction in a closed-tube format. The aim of this work is to assess the suitability and validity of the KASP method for detection in weedy rice of the three imidazolinone resistance mutations reported to date in rice.

Palabras-Clave: | CLEARFIELD RICE; DNA - BASED RESISTANCE DIAGNOSIS; HERBICIDE RESISTANCE; KASP; RED RICE; SNP.
Thesagro: | ARROZ ROJO; IMIDAZOLINONAS; RESISTENCIA A HERBICIDAS.
Categoría del tema: | F30 Genética vegetal y fitomejoramiento
Registro original: | INIA Treinta y Tres (TT)
Ejemplares: | TT - (100015-1) - PXI - AP - DD - PP/Rosas/Arb/2014/1 -  -  - DIS